Recombinant Anti-SRPK2 antibody [EPR16366] - BSA and Azide free (ab251113)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16366] to SRPK2 - BSA and Azide free
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
-
Product name
Anti-SRPK2 antibody [EPR16366] - BSA and Azide free
See all SRPK2 primary antibodies -
Description
Rabbit monoclonal [EPR16366] to SRPK2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
ab251113 is the carrier-free version of ab192238.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Clonality
Monoclonal -
Clone number
EPR16366 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab251113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa).
|
|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
ICC/IF |
Use at an assay dependent concentration.
|
Notes |
---|
WB
Use at an assay dependent concentration. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
Target
-
Function
Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Promotes neuronal apoptosis by up-regulating cyclin-D1 (CCND1) expression. This is done by the phosphorylation of SRSF2, leading to the suppression of p53/TP53 phosphorylation thereby relieving the repressive effect of p53/TP53 on cyclin-D1 (CCND1) expression. Phosphorylates ACIN1, and redistributes it from the nuclear speckles to the nucleoplasm, resulting in cyclin A1 but not cyclin A2 up-regulation. Plays an essential role in spliceosomal B complex formation via the phosphorylation of DDX23/PRP28. Can mediate hepatitis B virus (HBV) core protein phosphorylation. Plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles. -
Tissue specificity
Highly expressed in brain, moderately expressed in heart and skeletal muscle and at low levels in lung, liver, and kidney. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylation at Thr-492 by PKB/AKT1 enhances its stimulatory activity in triggering cyclin-D1 (CCND1) expression and promoting apoptosis in neurons, which can be blocked by YWHAB. It also enhances its protein kinase activity toward ACIN1 and SRSF2, promotes its nuclear translocation and prevents its proteolytic cleavage.
Proteolytically cleaved at Asp-139 and Asp-403 by caspase-3 during apoptotic cell death. Cleavage at Asp-139 which is the major site of cleavage, produces a small N-terminal fragment that translocates into nucleus and promotes VP16-induced apoptosis. -
Cellular localization
Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. KAT5/TIP60 inhibits its nuclear translocation. Phosphorylation at Thr-492 by PKB/AKT1 promotes nuclear translocation. - Information by UniProt
-
Database links
- Entrez Gene: 6733 Human
- Entrez Gene: 20817 Mouse
- Entrez Gene: 296753 Rat
- Omim: 602980 Human
- SwissProt: P78362 Human
- SwissProt: O54781 Mouse
- Unigene: 285197 Human
- Unigene: 288728 Mouse
-
Alternative names
- Human serine kinase SRPK2 mRNA, complete cds antibody
- Serine kinase SRPK2 antibody
- Serine/arginine rich protein specific kinase 2 antibody
see all
Images
-
All lanes : Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 115 kDaThis data was developed using ab192238, the same antibody clone in a different buffer formulation.
-
This data was developed using ab192238, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of Neuro-2a cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling SRPK2 with ab192238 at 1/250 dilution (5.4 μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Negative controls: anti-SRPK2 at 1/250 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/500 dilution. -
This data was developed using ab192238, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human endometrium adenocarcinoma tissue labeling SRPK2 with ab192238 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
This data was developed using ab192238, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SRPK2 with ab192238 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238) at 1/1000 dilution + Human fetal kidney tissue lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 115 kDaThis data was developed using ab192238, the same antibody clone in a different buffer formulation.
-
Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238) at 1/2000 dilution + Human fetal brain lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 115 kDaThis data was developed using ab192238, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab251113 has not yet been referenced specifically in any publications.