Anti-STK3/MST-2 antibody [3067C3a] (ab71960)
Key features and details
- Mouse monoclonal [3067C3a] to STK3/MST-2
- Suitable for: WB
- Knockout validated
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2b
Overview
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Product name
Anti-STK3/MST-2 antibody [3067C3a]
See all STK3/MST-2 primary antibodies -
Description
Mouse monoclonal [3067C3a] to STK3/MST-2 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human STK3/MST-2.
Database link: Q13188 -
Positive control
- WB: HeLa, HAP1 and NIH3T3 cell lysates; Recombinant human STK3/MST-2.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab71960 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane. -
Clonality
Monoclonal -
Clone number
3067C3a -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab71960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 56 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 56 kDa). |
Target
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Function
Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. MST1/MST2 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1. -
Tissue specificity
Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain.
Contains 1 SARAH domain. -
Cellular localization
Cytoplasm. Nucleus. The caspase-cleaved form cycles between nucleus and cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 6788 Human
- Omim: 605030 Human
- SwissProt: Q13188 Human
- Unigene: 492333 Human
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Alternative names
- 0610042I06Rik antibody
- EC 2.7.11.1 antibody
- FLJ90748 antibody
see all
Images
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All lanes : Anti-STK3/MST-2 antibody [3067C3a] (ab71960) at 1/10000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : STK3 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDaLanes 1- 2: Merged signal (red and green). Green - ab71960 observed at 56 kDa. Red - Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) observed at 37 kDa.
ab71960 was shown to react with STK3/MST-2 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265082 (knockout cell lysate ab257714) was used. Wild-type HeLa and STK3 knockout HeLa cell lysates were subjected to SDS-PAGE. ab71960 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at a 1 in 10000 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye®800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye®680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: STK3/MST-2 knockout HAP1 cell lysate (20 µg)
Lane 3: NIH/3T3 cell lysate (20 µg)
Lane 4: 293T cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab71960 observed at 56 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab71960 was shown to recognize STK3/MST-2 when STK3/MST-2 knockout samples were used, along with additional cross-reactive bands. Wild-type and STK3/MST-2 knockout samples were subjected to SDS-PAGE. ab71960 and ab181602 (loading control to STK3/MST-2) were diluted 1 μg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging. -
Anti-STK3/MST-2 antibody [3067C3a] (ab71960) + immunising recombinant protein
Predicted band size: 56 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
Datasheets and documents
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Datasheet download
References (1)
ab71960 has been referenced in 1 publication.
- Chatzifrangkeskou M et al. RASSF1A is required for the maintenance of nuclear actin levels. EMBO J 38:e101168 (2019). PubMed: 31414556