Overview

  • Product name
  • Description
    Rabbit polyclonal to SWAP70
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human SWAP70 aa 300-505.
    Sequence:

    A IHSTIHLLKL GSPPPHKEAR QRRKELRKKQ LAEQEELERQ MKELQAANES KQQELEAVRK KLEEAASRAA EEEKKRLQTQ VELQARFSTE LEREKLIRQQ MEEQVAQKSS ELEQYLQRVR ELEDMYLKLQ EALEDERQAR QDEETVRKLQ ARLLEEESSK RAELEKWHLE QQQAIQTTEA EKQELENQRV LKEQALQEAM EQLEQ


    Database link: Q9UH65

  • Positive control
    • Ramos and Raji cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab220176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 69 kDa.

Target

  • Function
    Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion.
  • Tissue specificity
    Expressed only in mature B-cells including those associated with mucosa-associated tissue and bronchus-associated tissue (PubMed:10681448). Widely expressed. Abundant in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes and macrophages (PubMed:12925760).
  • Sequence similarities
    Contains 1 PH domain.
  • Domain
    The PH domain is essential for phosphatidylinositol 3,4,5-trisphosphate binding.
  • Post-translational
    modifications
    Tyrosine-phosphorylated.
  • Cellular localization
    Cytoplasm. Cell membrane. Nucleus. Cell projection > lamellipodium. In resting B-cells it is localized mainly in the cytoplasm and upon cell activation it is recruited to the plasma membrane and then translocates to the nucleus. In activated, class-switching B-cells it is associated with membrane IgG but not IgM. Localized to loose actin filament arrays located behind actively extending lamellipodia.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA0640 antibody
    • 70kDa antibody
    • AV235546 antibody
    • FLJ39540 antibody
    • HSPC321 antibody
    • SWA 70 protein antibody
    • SWAP 70 antibody
    • SWAP switching B-cell complex 70kDa subunit antibody
    • SWAP-70 antibody
    • SWAP70 antibody
    • Switch associated protein 70 antibody
    • Switch-associated protein 70 antibody
    • SWP70_HUMAN antibody
    see all

Images

  • All lanes : Anti-SWAP70 antibody (ab220176) at 4 µg/ml

    Lane 1 : Raji cell lysate
    Lane 2 : Ramos cell lysate

    Predicted band size: 69 kDa

References

ab220176 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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