Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)
Key features and details
- Mouse monoclonal [EA-53] to Sarcomeric Alpha Actinin
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Sarcomeric Alpha Actinin antibody [EA-53]
See all Sarcomeric Alpha Actinin primary antibodies -
Description
Mouse monoclonal [EA-53] to Sarcomeric Alpha Actinin -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Rabbit -
Immunogen
Full length native protein (purified) corresponding to Rabbit Sarcomeric Alpha Actinin. Purified rabbit skeletal α-actinin.
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Positive control
- WB: Mouse and rat skeletal muscle and rat heart tissue lysates. IHC-P: Human skeletal muscle tissue.
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General notes
This product was changed from ascites to tissue culture supernatant on 9th September 2019. Lot numbers higher than GR3258034 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Please see notes section. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Proprietary Purification -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
EA-53 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 10 - 20 µg/ml.
ABC method. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation and dilutions should be determined by the user. |
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WB | (2) |
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
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Notes |
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IHC-P
Use a concentration of 10 - 20 µg/ml. ABC method. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation and dilutions should be determined by the user. |
WB
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa). |
Target
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Function
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. -
Tissue specificity
Expressed in both skeletal and cardiac muscle. -
Involvement in disease
Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
Sequence similarities
Belongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats. -
Cellular localization
Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle. - Information by UniProt
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Database links
- Entrez Gene: 88 Human
- Entrez Gene: 291245 Rat
- Omim: 102573 Human
- SwissProt: P35609 Human
- Unigene: 498178 Human
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Alternative names
- Actin binding protein antibody
- Actinin alpha 2 antibody
- ACTN 2 antibody
see all
Images
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Lane 1 : Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) at 1/500 dilution
Lane 2 : Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) at 1/1000 dilution
Lane 3 : Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) at 1/2000 dilution
Lane 4 : No antibody control
All lanes : Rat skeletal muscle extract
Secondary
All lanes : Goat Anti-Mouse IgG (Fab) (HRP) at 1/4000 dilution
Predicted band size: 103 kDa -
Immunohistochemistry formalin-fixed, paraffin-embedded human tongue labeling Alpha Actinin with ab9465 at 12 μg/mL.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (236)
ab9465 has been referenced in 236 publications.
- Mesquita FCP et al. Decellularized Extracellular Matrix Powder Accelerates Metabolic Maturation at Early Stages of Cardiac Differentiation in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Cells Tissues Organs 212:32-44 (2023). PubMed: 34933302
- Hagihara K et al. Gosha-jinki-Gan (GJG) shows anti-aging effects through suppression of TNF-α production by Chikusetsusaponin V. Gene 815:146178 (2022). PubMed: 34995733
- Skorska A et al. Monitoring the maturation of the sarcomere network: a super-resolution microscopy-based approach. Cell Mol Life Sci 79:149 (2022). PubMed: 35199227
- Huang M et al. Regulation of Myogenesis by a Na/K-ATPase α1 Caveolin-Binding Motif. Stem Cells 40:133-148 (2022). PubMed: 35257186
- Han Y et al. Loss of m6A Methyltransferase METTL5 Promotes Cardiac Hypertrophy Through Epitranscriptomic Control of SUZ12 Expression. Front Cardiovasc Med 9:852775 (2022). PubMed: 35295259