Anti-Serum Amyloid A antibody [115] (ab687)
Key features and details
- Mouse monoclonal [115] to Serum Amyloid A
- Suitable for: WB, Sandwich ELISA
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Serum Amyloid A antibody [115]
See all Serum Amyloid A primary antibodies -
Description
Mouse monoclonal [115] to Serum Amyloid A -
Host species
Mouse -
Tested applications
Suitable for: WB, Sandwich ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Serum Amyloid A. Highly purified recombinant human serum amyloid A (MW: 12 kDa).
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General notes
This product was changed from ascites to tissue culture supernatant on 6th March 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading...
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Purification notes
Affinity purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
115 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab687 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (4) |
Use at an assay dependent concentration.
Use at an assay dependent dilution. This antibody, at a concentration of 0.1-0.5 µg/ml, will allow visualisation of 100 ng/lane of recombinant human SAA. |
Sandwich ELISA |
Use at an assay dependent concentration.
sELISA compatible pair - ab18711. |
Notes |
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WB
Use at an assay dependent concentration. Use at an assay dependent dilution. This antibody, at a concentration of 0.1-0.5 µg/ml, will allow visualisation of 100 ng/lane of recombinant human SAA. |
Sandwich ELISA
Use at an assay dependent concentration. sELISA compatible pair - ab18711. |
Target
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Relevance
Function: Major acute phase reactant. Apolipoprotein of the HDL complex. Tissue specificity: Expressed by the liver; secreted in plasma. Disease: Note=Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function. Note=Elevated serum SAA protein levels may be associated with lung cancer. Similarity: Belongs to the SAA family. PTM: This protein is the precursor of amyloid protein A, which is formed by the removal of approximately 24 residues from the C-terminal end. -
Database links
- Entrez Gene: 694827 Human
- Omim: 104750 Human
- SwissProt: P0DJI8 Human
- SwissProt: P0DJI9 Human
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Alternative names
- Amyloid fibrils antibody
- SAA 2 antibody
- Serum amyloid A1 isoform 2 antibody
see all
Images
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All lanes : Anti-Serum Amyloid A antibody [115] (ab687) at 1/4000 dilution
Lanes 1-4 : Acute phase plasma samples
Lane 5 : Recombinant C-SAA
Lane 6 : Recombinant A-SAA
Lysates/proteins at 14 µg per lane.
Secondary
All lanes : Goat anti-mouse IgG-HRP at 1/10000 dilution
Developed using the ECL technique.
Exposure time: 5 seconds -
Anti-Serum Amyloid A antibody [115] (ab687) at 0.5 µg/ml +
Recombinant human Serum Amyloid A (mutated D60N + H71R) protein (ab50232) at 0.01 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1 minute
Protocols
Datasheets and documents
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Datasheet download
References (8)
ab687 has been referenced in 8 publications.
- Niu X et al. Serum amyloid A 1 induces suppressive neutrophils through the Toll-like receptor 2-mediated signaling pathway to promote progression of breast cancer. Cancer Sci 113:1140-1153 (2022). PubMed: 35102665
- Nakayama Y et al. Spontaneous, Experimentally Induced, and Transmissible AA Amyloidosis in Japanese Quail ( Coturnix japonica). Vet Pathol 54:912-921 (2017). PubMed: 28812532
- Inoue M et al. Significance of downregulation of liver fatty acid-binding protein in hepatocellular carcinoma. World J Gastroenterol 20:17541-51 (2014). PubMed: 25516669
- Jahangiri A et al. Serum amyloid A is found on ApoB-containing lipoproteins in obese humans with diabetes. Obesity (Silver Spring) 21:993-6 (2013). PubMed: 23784902
- Heo SH et al. Comparative proteomic analysis in children with idiopathic short stature (ISS) before and after short-term recombinant human growth hormone (rhGH) therapy. Proteomics 13:1211-9 (2013). Human . PubMed: 23436750
- Kramer HB et al. Elevation of intact and proteolytic fragments of acute phase proteins constitutes the earliest systemic antiviral response in HIV-1 infection. PLoS Pathog 6:e1000893 (2010). WB ; Human . PubMed: 20463814
- Christenson K et al. Serum amyloid A inhibits apoptosis of human neutrophils via a P2X7-sensitive pathway independent of formyl peptide receptor-like 1. J Leukoc Biol 83:139-48 (2008). PubMed: 17962368
- Rossi L et al. Inflammatory protein profile during systemic high dose interleukin-2 administration. Proteomics 6:709-20 (2006). PubMed: 16342236