Sheep Anti-Cow IgA H&L (HRP) (ab112755)
Key features and details
- Sheep Anti-Cow IgA H&L (HRP)
- Conjugation: HRP
- Host species: Sheep
- Isotype: IgG
- Suitable for: WB, ELISA, IHC-P, ICC/IF
Overview
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Product name
Sheep Anti-Cow IgA H&L (HRP)
See all IgA secondary antibodies -
Host species
Sheep -
Target species
Cow -
Specificity
By immunoelectrophoresis and ELISA ab112755 reacts specifically with bovine IgA. This antibody may cross react with IgA from other species. -
Tested applications
Suitable for: WB, ELISA, IHC-P, ICC/IFmore details -
Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was solid phase adsorbed to ensure class specificity. ab112755 was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP). -
Conjugation notes
Molar enzyme/antibody protein ratio is 4:1. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab112755 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. with colorimetric detection. Use at 1/1000 - 1/30000 with chemiluminescent detection.
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ELISA |
1/10000 - 1/100000. as primary antibody.
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IHC-P |
1/200 - 1/500.
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ICC/IF |
1/200 - 1/500.
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Notes |
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WB
1/1000 - 1/10000. with colorimetric detection. Use at 1/1000 - 1/30000 with chemiluminescent detection. |
ELISA
1/10000 - 1/100000. as primary antibody. |
IHC-P
1/200 - 1/500. |
ICC/IF
1/200 - 1/500. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab112755 has not yet been referenced specifically in any publications.