Anti-SODD antibody (ab2048)
Key features and details
- Rabbit polyclonal to SODD
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SODD antibody
See all SODD primary antibodies -
Description
Rabbit polyclonal to SODD -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human SODD aa 2-16.
Sequence:SALRRSGYGPSDGPSC
(Peptide available asab8382) -
Positive control
- HeLa whole cell lysate or THP-1 whole cell lysate.
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General notes
Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNF-R1 and Fas. Several novel death receptors including DR3, DR4, DR5, and DR6 were recently identified. Cell death signal is transduced by death domain containing adapter molecules through the interaction with death domain of these death receptors. A novel TNF-R1 interacting protein was recently identified and designated SODD for SODDs. SODD associates with the death domain of TNF-R1 and prevents constitutive activation of TNF-R1 signaling. TNF treatment releases SODD and permits adapter molecules such as TRADD recruiting to the active TNF-R1 complex, which activates TNF signaling pathways. SODD also interacts with DR3. SODD is ubiquitously expressed in human tissues and cell lines.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
DEAE-Chromatography -
Purification notes
SODD Antibody is Antibody is DEAE purified. -
Primary antibody notes
Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNF-R1 and Fas. Several novel death receptors including DR3, DR4, DR5, and DR6 were recently identified. Cell death signal is transduced by death domain containing adapter molecules through the interaction with death domain of these death receptors. A novel TNF-R1 interacting protein was recently identifiedand designated SODD for silencer of death domains. SODD associates with the death domain of TNF-R1 and prevents constitutive activation of TNF-R1 signaling. TNF treatment releases SODD and permits adapter molecules such as TRADD recruiting to the active TNF-R1 complex, which activates TNF signaling pathways. SODD also interacts with DR3. SODD is ubiquitously expressed in human tissues and cell lines. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 5 µg/ml.
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WB |
1/500 - 1/2000. Detects a band of approximately 60 kDa.
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Notes |
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ICC/IF
Use a concentration of 5 µg/ml. |
WB
1/500 - 1/2000. Detects a band of approximately 60 kDa. |
Target
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Function
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release (By similarity). Prevents constitutive TNFRSF1A signaling. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Contains 1 BAG domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 9530 Human
- Omim: 603884 Human
- SwissProt: O95429 Human
- Unigene: 194726 Human
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Alternative names
- BAG 4 antibody
- BAG family molecular chaperone regulator 4 antibody
- BAG-4 antibody
see all
Images
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All lanes : Anti-SODD antibody (ab2048) at 1/500 dilution
Lane 1 : HeLa whole cell lysate with absence of blocking peptide
Lane 2 : THP-1 whole cell lysate with absence of blocking peptide
Lane 3 : HeLa whole cell lysate with SODD peptide (ab8382)
Lane 4 : THP-1 whole cell lysate with SODD peptide (ab8382)
Observed band size: 60 kDa why is the actual band size different from the predicted? -
ab2048 at 5µg/ml staining SODD in Hela cells by ICC/IF
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Immunofluorescence of SODD in Hela cells using ab2048 at 20 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab2048 has been referenced in 2 publications.
- Yi L et al. Bcl-2 associated athanogene 4 promotes proliferation, migration and invasion of gastric cancer cells. Mol Med Rep 16:3753-3760 (2017). PubMed: 29067445
- Boiani M et al. The stress protein BAG3 stabilizes Mcl-1 protein and promotes survival of cancer cells and resistance to antagonist ABT-737. J Biol Chem 288:6980-90 (2013). WB ; Human . PubMed: 23341456