Superoxide Dismutase Activity Assay Kit (Colorimetric) (ab65354)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Cell culture media, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
Overview
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Product name
Superoxide Dismutase Activity Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media -
Assay type
Enzyme activity -
Assay time
0h 30m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Superoxide Dismutase Activity Assay Kit (Colorimetric) ab65354 is a simple and rapid assay for superoxide dismutase (SOD) activity.
In the SOD assay protocol:
- superoxide anions are produced by the action of xanthine oxidase
- SOD catalyzes the dismutation of the superoxide anion into hydrogen peroxide and O2
- superoxide anions act on WST-1 to produce a water-soluble formazan dye which can be detected by the increase in absorbance at 450 nm
The greater the activity of SOD in the sample, the less formazan dye is produced.
Superoxide dismutase assay protocol summary:
- add samples to wells
- add WST-1 working solution and enzyme working solution and incubate for 20 min at 37ºC
- analyze with microplate reader -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K335 Superoxide Dismutase (SOD) Activity Colorimetric Assay Kit. K335-100 is the same size as the 100 test size of ab65354.
Superoxide dismutase (SOD) is one of the most important antioxidative enzymes. It catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen.
Related products
Review the oxidative stress marker and assay guide to learn about more assays for oxidative stress.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 2000 tests SOD Assay Buffer 1 x 20ml 20 x 20ml SOD Dilution Buffer 1 x 10ml 20 x 10ml SOD Enzyme Solution 1 vial 20 vials WST Reagent II 1 x 1ml 20 x 1ml -
Research areas
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Relevance
Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn SOD/SOD1), mitochondrial manganese SOD (Mn SOD/SOD2) and extracellular Cu, Zn SOD (EC SOD/SOD3). -
Cellular localization
Cytoplasmic -
Alternative names
- ALS
- ALS1
- Cu /Zn superoxide dismutase
see all
Associated products
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Related Products
Images
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Park J et al investigates the recovery in erectile function after administration of chronic statin alone in DM (streptozotocin (STZ)-induced diabetes mellitus) rats. SOD activity was determined using Superoxide Dismutase activity assay kit (ab65354).
* Indicates statistical significance in comparison with DM group (P < 0.05). # Indicates statistical significance in comparison with the statin group (P<0.05).
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Superoxide dismutase measured in biofluids at various dilutions
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Superoxidase dismutase (ab90040) measured showing inhibition rate (%) per concentration (microgram per mL)
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Principle of Superoxide Dismutase Assay.
Datasheets and documents
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SDS download
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Datasheet download
References (242)
ab65354 has been referenced in 242 publications.
- Hoque MR et al. Effect of Detoxified Nano Sulfur Supplementation on the Growth, Nutrient Digestibility, Meat Quality, Excreta Microbes, Gas Emissions, and Blood Profiles of Broilers. J Poult Sci 59:48-55 (2022). PubMed: 35125912
- Huang C et al. Cardioprotective Effect of circ_SMG6 Knockdown against Myocardial Ischemia/Reperfusion Injury Correlates with miR-138-5p-Mediated EGR1/TLR4/TRIF Inactivation. Oxid Med Cell Longev 2022:1927260 (2022). PubMed: 35126807
- Zhu Y et al. miR-340-5p Mediates Cardiomyocyte Oxidative Stress in Diabetes-Induced Cardiac Dysfunction by Targeting Mcl-1. Oxid Med Cell Longev 2022:3182931 (2022). PubMed: 35126811
- Won EJ et al. Molecular evidence for suppression of swimming behavior and reproduction in the estuarine rotifer Brachionus koreanus in response to COVID-19 disinfectants. Mar Pollut Bull 175:113396 (2022). PubMed: 35149311
- Farooqi HMU et al. Study of the Anticancer Potential of Plant Extracts Using Liver Tumor Microphysiological System. Life (Basel) 12:N/A (2022). PubMed: 35207423