Product nameAnti-Synapsin I antibody - Synaptic Marker
See all Synapsin I primary antibodies
DescriptionRabbit polyclonal to Synapsin I - Synaptic Marker
Tested applicationsSuitable for: WB, IHC-P, IHC-FoFr, ICC/IF, IPmore details
Species reactivityReacts with: Mouse, Rat, Sheep, Goat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Rat Synapsin I aa 600-700 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
(Peptide available as
- WB: Mouse spinal cord, mouse brain and rat brain tissue lysates. IP: Mouse brain tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab64581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 74, 70 kDa (predicted molecular weight: 74 kDa).
Abcam recommends using 3-5% milk as the blocking agent.
|IHC-P||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
FunctionNeuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level.
Involvement in diseaseDefects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders [MIM:300491]. XELBD is characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior.
Sequence similaritiesBelongs to the synapsin family.
modificationsSubstrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCell junction > synapse. Golgi apparatus.
- Information by UniProt
- Brain protein 4.1 antibody
- SYN 1 antibody
- SYN 1a antibody
IHC-P image of ab64581 staining of Synapsin 1 in lamb cerebellum. Heat mediated antigen retrieval buffer was performed using citric acid buffer. The sections were then incubated in 1% BSA to block non-specific protein-protein interactions. The sections were incubated with ab64581 (1:100) for two hours at +21°C, followed by biotin conjugated secondary antibody. There appears to be appropriate, identical positivity in lamb, goat, mouse and rat brain.
Anti-Synapsin I antibody - Synaptic Marker (ab64581) at 1/5000 dilution + Rat whole brain tissue lysate at 15 µg
Donkey anti-Rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 80,85 kDa why is the actual band size different from the predicted?
Exposure time: 15 seconds
Blocked with 5% milk for 1 hour at 25°C.
Sample was incubated with primary antibody for 18 hours at 4°C in TBS + 0.1% Tween.
ab64581 staining cultured rat hippocampal neurons by ICC/IF. The cultured neurons were fixed with methanol, permeabilized with 0.3% Triton X in 0.1% PBS and blocked with 10% donkey serum for 30 minutes at 24°C. The cultured neurons were then stained with ab64581 at 1/200 in 0.3% TritonX with 0.1% PBS and 10% donkey serum for 4h at 24°C. An Alexa Fluro 488 donkey anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei were stained with Hoechst and can be observed in blue.
All lanes : Anti-Synapsin I antibody - Synaptic Marker (ab64581) at 1 µg/ml
Lane 1 : Spinal Cord (Mouse) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate - normal tissue
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 70,74 kDa why is the actual band size different from the predicted?
The immunogen for ab64581 is predicted to recognise both the 70 kDa and 74 kDa isoforms of Synapsin I. Synapsin I is also known to contain a number of potential phosphorylation and glycosylation sites (SwissProt data).
ab64581 staining Synapsin I in rat brain (cerebellum) tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.01% Triton X-100, blocked with 10% serum for 2 hours at 25°C; antigen retrieval was by heat mediation in a citrate buffer. The sample was incubated with the primary antibody (1/300) at 25°C for 12 hours. An undiluted Alexa Fluor® 555-conjugated goat anti-rabbit polyclonal was used as the secondary antibody.
Synapsin I - Synaptic Marker was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to Synapsin I - Synaptic Marker and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab64581.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 80kDa: Synapsin I - Synaptic Marker contains a number of potential glycosylation sites (SwissProt) which may explain the higher migrating band.
This product has been referenced in:
- Cifre M et al. Cognitive impairment in metabolically-obese, normal-weight rats: identification of early biomarkers in peripheral blood mononuclear cells. Mol Neurodegener 13:14 (2018). Read more (PubMed: 29566703) »
- Stoffel W et al. SMPD3 deficiency perturbs neuronal proteostasis and causes progressive cognitive impairment. Cell Death Dis 9:507 (2018). IHC ; Mouse . Read more (PubMed: 29725009) »