Anti-T Plastin/PLS3 antibody (ab137585)
Key features and details
- Rabbit polyclonal to T Plastin/PLS3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-T Plastin/PLS3 antibody
See all T Plastin/PLS3 primary antibodies -
Description
Rabbit polyclonal to T Plastin/PLS3 -
Host species
Rabbit -
Specificity
ab137585 may also detect Plastin L as the sequence shows 82% identity. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Xenopus laevis, Zebrafish, Xenopus tropicalis -
Immunogen
Recombinant fragment corresponding to Human T Plastin/PLS3 aa 66-371.
Database link: P13797 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137585 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
1/500 - 1/3000. Predicted molecular weight: 71 kDa.
|
ICC/IF |
1/100 - 1/1000.
|
Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 71 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. -
Tissue specificity
Expressed in a variety of organs, including muscle, brain, uterus and esophagus. -
Sequence similarities
Contains 2 actin-binding domains.
Contains 4 CH (calponin-homology) domains.
Contains 2 EF-hand domains. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5358 Human
- Entrez Gene: 102866 Mouse
- Entrez Gene: 81748 Rat
- Omim: 300131 Human
- SwissProt: P13797 Human
- SwissProt: Q99K51 Mouse
- SwissProt: Q63598 Rat
- Unigene: 496622 Human
see all -
Alternative names
- BMND18 antibody
- Plastin 3 (T isoform) antibody
- Plastin 3 antibody
see all
Images
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All lanes : Anti-T Plastin/PLS3 antibody (ab137585) at 1/500 dilution
Lane 1 : HeLa S3 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 71 kDa
7.5% SDS PAGE -
All lanes : Anti-T Plastin/PLS3 antibody (ab137585) at 1/1000 dilution
Lane 1 : NIH-3T3 whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL-1 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 71 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of methanol-fixed HeLa cells labelling T Plastin/PLS3 with ab137585 at 1/200 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab137585 has been referenced in 7 publications.
- Wen Q et al. Myosin VIIa Supports Spermatid/Organelle Transport and Cell Adhesion During Spermatogenesis in the Rat Testis. Endocrinology 160:484-503 (2019). PubMed: 30649248
- Wen Q et al. Actin nucleator Spire 1 is a regulator of ectoplasmic specialization in the testis. Cell Death Dis 9:208 (2018). WB . PubMed: 29434191
- Gao Y et al. Perfluorooctanesulfonate (PFOS)-induced Sertoli cell injury through a disruption of F-actin and microtubule organization is mediated by Akt1/2. Sci Rep 7:1110 (2017). WB ; Rat . PubMed: 28439067
- Gao Y et al. Regulation of the blood-testis barrier by a local axis in the testis: role of laminin a2 in the basement membrane. FASEB J 31:584-597 (2017). PubMed: 27815338
- Gao Y et al. Polarity protein Crumbs homolog-3 (CRB3) regulates ectoplasmic specialization dynamics through its action on F-actin organization in Sertoli cells. Sci Rep 6:28589 (2016). PubMed: 27358069
- Li N et al. Overexpression of plastin 3 in Sertoli cells disrupts actin microfilament bundle homeostasis and perturbs the tight junction barrier. Spermatogenesis 6:e1206353 (2016). PubMed: 27559491
- Li A et al. Fascin is regulated by slug, promotes progression of pancreatic cancer in mice, and is associated with patient outcomes. Gastroenterology 146:1386-96.e1-17 (2014). Mouse . PubMed: 24462734