Anti-TAF4B antibody - C-terminal (ab189428)
Key features and details
- Rabbit polyclonal to TAF4B - C-terminal
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-TAF4B antibody - C-terminal -
Description
Rabbit polyclonal to TAF4B - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human TAF4B aa 650-750 (C terminal) conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q92750 -
Positive control
- WB: K562 cell lysate. IHC-P: Human liver tissue. Flow Cyt (Intra): K562 cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000. Predicted molecular weight: 91 kDa.
|
|
IHC-P |
Use a concentration of 5 µg/ml.
|
Notes |
---|
Flow Cyt (Intra)
1/10 - 1/50. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000. Predicted molecular weight: 91 kDa. |
IHC-P
Use a concentration of 5 µg/ml. |
Target
-
Relevance
TAF4B is a cell type-specific subunit of TFIID that may function as a gene-selective coactivator in certain cells. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. It is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). This protein is preferentially expressed in ovarian granulosa cells and is highly expressed in B cells. -
Cellular localization
Nucleus. Cytoplasm. Export into the cytoplasm is mediated by a CRM1-independent nuclear export pathway and not by phosphorylation. -
Database links
- Entrez Gene: 6875 Human
- Omim: 601689 Human
- SwissProt: Q92750 Human
-
Alternative names
- TAF(II)105 antibody
- TAF2C2 antibody
- TAF4b RNA polymerase II, TATA box binding protein (TBP) associated factor, 105kDa antibody
see all
Images
-
Anti-TAF4B antibody - C-terminal (ab189428) at 1/1000 dilution + K562 cell lysate at 35 µg
Predicted band size: 91 kDa -
Intracellular flow cytometric analysis of K562 cells labeling TAF4B with ab189428 at 1/10 dilution (right histogram) compared to negative control cells (left histogram) using FITC-conjugated goat-anti-rabbit secondary antibodies.
-
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labeling TAF4B with ab189428 at 5 μg/ml.
Protocols
Datasheets and documents
-
Datasheet download
References (0)
ab189428 has not yet been referenced specifically in any publications.