Anti-TAF6 antibody [585D4a] (ab51026)
Key features and details
- Mouse monoclonal [585D4a] to TAF6
- Suitable for: WB, IP, ICC
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-TAF6 antibody [585D4a]
See all TAF6 primary antibodies -
Description
Mouse monoclonal [585D4a] to TAF6 -
Host species
Mouse -
Tested applications
Suitable for: WB, IP, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to TAF6.
Database link: P49848-1 -
Positive control
- HEK293 whole cell lysate. HeLa cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified using protein G chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 micrometer membrane. -
Clonality
Monoclonal -
Clone number
585D4a -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/50. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
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IP |
Use at 10 µg/mg of lysate.
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ICC |
1/5.
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Notes |
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WB
1/50. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa). |
IP
Use at 10 µg/mg of lysate. |
ICC
1/5. |
Target
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Relevance
TAF6 is a subunit of transcription factor IID (TFIID). Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. TAF6 is one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. Four isoforms have been identified but complete transcripts have been determined for only three isoforms. One of the isoforms has been shown to preclude binding of one of the other TFIID subunits, thereby reducing transcription and initiating signals that trigger apoptosis. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 6878 Human
- Omim: 602955 Human
- SwissProt: P49848 Human
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Alternative names
- TAF2E antibody
- E 70/85kD antibody
- TAF 6 antibody
see all
Images
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Anti-TAF6 antibody [585D4a] (ab51026) at 1/50 dilution + HEK293 whole cell lysate at 25 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 73 kDa
Observed band size: 73 kDa
Additional bands at: 105 kDa. We are unsure as to the identity of these extra bands. -
ab51026 was used at 5µg/500µg of RIPA lysate of HeLa cells. Western blot was labelled with ab51026 at 1/50 dilution followed by Mouse IgG antibody at 1/2500 dilution and developed using the ECL technique.
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HeLa cells labelled with ab51026 at 1/5 dilution. Alexa Fluor 488 Goat anti-Mouse IgG was used as secondary antibody at 1/200 dilution.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab51026 has been referenced in 2 publications.
- Sela D et al. Endoplasmic Reticulum Stress-Responsive Transcription Factor ATF6a Directs Recruitment of the Mediator of RNA Polymerase II Transcription and Multiple Histone Acetyltransferase Complexes. J Biol Chem : (2012). WB . PubMed: 22577136
- Takahashi H et al. Proteomics Reveals a Physical and Functional Link between Hepatocyte Nuclear Factor 4{alpha} and Transcription Factor IID. J Biol Chem 284:32405-12 (2009). WB ; Human . PubMed: 19805548