Product nameAnti-TAPP-2 antibody
DescriptionRabbit polyclonal to TAPP-2
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human TAPP-2 aa 52-189.
- Human Pancreas tissue; RT-4 and U-251 MG cell lysate; Human Tonsil lysate.
Previously labelled as PLEKHA2.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121218 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/250 - 1/500.|
FunctionBinds specifically to phosphatidylinositol-3,4-diphosphate (PtdIns3,4P2), but not to other phosphoinositides. May recruit other proteins to the plasma membrane.
Tissue specificityHighly expressed in heart, kidney, spleen and peripheral blood leukocytes. Detected at lower levels in brain, skeletal muscle, colon, thymus, liver, small intestine, placenta and lung.
Sequence similaritiesContains 2 PH domains.
Cellular localizationCytoplasm. Cell membrane. Nucleus. Locates to the plasma membrane after treatments that stimulate the production of PtdIns3,4P2.
- Information by UniProt
- PH domain-containing family A member 2 antibody
- PKHA2_HUMAN antibody
- Pleckstrin homology domain-containing family A member 2 antibody
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus and cytoplasm. Recommended concentration of ab121218 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121218, at 1/200 dilution, staining TAPP-2 in paraffin-embedded Human pancreas tissue, by Immunohistochemistry.
All lanes : Anti-TAPP-2 antibody (ab121218) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique.
ab121218 has not yet been referenced specifically in any publications.