Anti-TATA binding protein TBP antibody - Loading Control (ab74222)
Key features and details
- Rabbit polyclonal to TATA binding protein TBP - Loading Control
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-TATA binding protein TBP antibody - Loading Control
See all TATA binding protein TBP primary antibodies -
Description
Rabbit polyclonal to TATA binding protein TBP - Loading Control -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Snake, Rhesus monkey, Gorilla, Orangutan, Platypus -
Immunogen
Synthetic peptide corresponding to Human TATA binding protein TBP (N terminal).
Database link: P20226 -
Positive control
- HeLa or 293T whole cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 38 kDa.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 38 kDa. |
Target
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Function
General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. -
Tissue specificity
Widely expressed, with levels highest in the testis and ovary. -
Involvement in disease
Defects in TBP are the cause of spinocerebellar ataxia type 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease. -
Sequence similarities
Belongs to the TBP family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 395995 Chicken
- Entrez Gene: 431689 Dog
- Entrez Gene: 6908 Human
- Entrez Gene: 21374 Mouse
- Entrez Gene: 117526 Rat
- Omim: 600075 Human
- SwissProt: O13270 Chicken
- SwissProt: P20226 Human
see all -
Alternative names
- GTF2D antibody
- GTF2D1 antibody
- HDL4 antibody
see all
Images
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All lanes : Anti-TATA binding protein TBP antibody - Loading Control (ab74222) at 1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 38 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (1)
ab74222 has been referenced in 1 publication.
- Perdigones N et al. Clonal hematopoiesis in patients with dyskeratosis congenita. Am J Hematol 91:1227-1233 (2016). PubMed: 27622320