Biotin Anti-TCR gamma + TCR delta antibody [UC7-13D5] (ab25209)
Key features and details
- Biotin Armenian hamster monoclonal [UC7-13D5] to TCR gamma + TCR delta
- Suitable for: IHC-Fr, IP, Flow Cyt, Functional Studies
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Biotin Anti-TCR gamma + TCR delta antibody [UC7-13D5]
See all TCR gamma + TCR delta primary antibodies -
Description
Biotin Armenian hamster monoclonal [UC7-13D5] to TCR gamma + TCR delta -
Host species
Armenian hamster -
Conjugation
Biotin -
Specificity
ab25209 is specific for the gamma-delta heterodimer and plate-bound ab25209 activates gamma-delta TCR bearing cells. This antibody does not react with alpha-beta TCR-expressing T cells. -
Tested applications
Suitable for: IHC-Fr, IP, Flow Cyt, Functional Studiesmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to TCR gamma + TCR delta. Mouse T cell clone G8
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
UC7-13D5 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25209 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
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Functional Studies |
Use at an assay dependent concentration.
In vitro activation of gamma-delta TCR-bearing cells and depletion of gamma-delta TCR-expressing cells. |
Notes |
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IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
Functional Studies
Use at an assay dependent concentration. In vitro activation of gamma-delta TCR-bearing cells and depletion of gamma-delta TCR-expressing cells. |
Target
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Relevance
T cell receptors (TCR) recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one a and one b chain or one d and one g chain. This region represents the germline organization of the T cell receptor beta locus. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. During T cell development, the beta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment; a V segment is then joined to the D-J gene. The C segment is later joined by splicing at the RNA level. The g/d TCR associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most g/d T cells are CD4-/CD8-, some are CD8+. T cells expressing the g/d TCR have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease. -
Cellular localization
Type I membrane protein -
Alternative names
- CD_antigen=CD3d antibody
- CD3D antibody
- CD3G antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab25209 has been referenced in 1 publication.
- Dent AL et al. Self-reactive gamma delta T cells are eliminated in the thymus. Nature 343:714-9 (1990). PubMed: 1968227