Recombinant Anti-TDG antibody [EPR8774] (ab154192)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8774] to TDG
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-TDG antibody [EPR8774]
See all TDG primary antibodies -
Description
Rabbit monoclonal [EPR8774] to TDG -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human TDG.
Database link: Q13569 -
Positive control
- Fetal lung tissue, Jurkat cell lysate, HeLa cells and HeLa cell lysate, K562 cell lysate and Fetal brain lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8774 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154192 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 46 kDa.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 46 kDa. |
Target
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Function
In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. This enzyme corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine. -
Sequence similarities
Belongs to the TDG/mug DNA glycosylase family. -
Post-translational
modificationsSumoylation on Lys-330 by either SUMO1 or SUMO2 induces dissociation of the product DNA. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6996 Human
- Entrez Gene: 21665 Mouse
- Entrez Gene: 114521 Rat
- Omim: 601423 Human
- SwissProt: Q13569 Human
- SwissProt: P56581 Mouse
- Unigene: 584809 Human
- Unigene: 284252 Mouse
see all -
Alternative names
- C JUN leucine zipper interactive protein antibody
- C JUN leucine zipper interactive protein antibody
- C-JUN leucine zipper interactive protein JZA-3 antibody
see all
Images
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All lanes : Anti-TDG antibody [EPR8774] (ab154192) at 1/5000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse spleen tissue lysate
Lane 5 : Rat brain tissue lysate
Lane 6 : Rat heart tissue lysate
Lane 7 : Rat kidney tissue lysate
Lane 8 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 46 kDa
Observed band size: 55-60 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
All lanes : Anti-TDG antibody [EPR8774] (ab154192) at 1/1000 dilution
Lane 1 : Fetal lung cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : K562 cell lysate
Lane 5 : Fetal brain cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 46 kDa -
Intracellular flow cytometric analysis of permeabilized HeLa cells labeling TDG with ab154192 at 1/10 dilution (red) compared to a negative control rabbit IgG antibody (green).
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab154192 has been referenced in 3 publications.
- Nanan KK et al. TET-Catalyzed 5-Carboxylcytosine Promotes CTCF Binding to Suboptimal Sequences Genome-wide. iScience 19:326-339 (2019). PubMed: 31404833
- Wu X et al. Porcine Circovirus Type 2 Rep Enhances IL-10 Production in Macrophages via Activation of p38-MAPK Pathway. Viruses 11:N/A (2019). PubMed: 31835539
- Neri F et al. Single-Base Resolution Analysis of 5-Formyl and 5-Carboxyl Cytosine Reveals Promoter DNA Methylation Dynamics. Cell Rep N/A:N/A (2015). PubMed: 25660018