Overview

  • Product name
  • Description
    Rabbit polyclonal to TGF beta 1
  • Host species
    Rabbit
  • Specificity
    Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.
  • Tested applications
    Suitable for: WB, IHC-FrFl, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Synthetic peptide corresponding to Human TGF beta 1. Surrounding amino acid 374.

  • Positive control
    • WB: RAW 264.7 whole cell lysate (ab7187), Mouse 3T3 cell lysate. IHC-P: Mouse intestine tissue; human retina tissue.
  • General notes

    Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).

    See other anti-rabbit secondary antibodies that can be used with this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab92486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 44 kDa.Can be blocked with Human TGF beta 1 peptide (ab231650).

Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.

IHC-FrFl Use at an assay dependent concentration. PubMed: 24647450
IHC-P Use a concentration of 10 - 20 µg/ml.
IHC-Fr Use a concentration of 10 - 20 µg/ml.
ICC/IF 1/100.

Target

  • Function
    Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
  • Tissue specificity
    Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
  • Involvement in disease
    Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
  • Sequence similarities
    Belongs to the TGF-beta family.
  • Post-translational
    modifications
    Glycosylated.
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cartilage-inducing factor antibody
    • CED antibody
    • Differentiation inhibiting factor antibody
    • DPD1 antibody
    • LAP antibody
    • Latency-associated peptide antibody
    • Prepro transforming growth factor beta 1 antibody
    • TGF beta 1 antibody
    • TGF beta antibody
    • TGF beta 1 protein antibody
    • TGF-beta 1 protein antibody
    • TGF-beta-1 antibody
    • TGF-beta-5 antibody
    • TGF-beta1 antibody
    • TGFB antibody
    • Tgfb-1 antibody
    • tgfb1 antibody
    • TGFB1_HUMAN antibody
    • TGFbeta antibody
    • TGFbeta1 antibody
    • Transforming Growth Factor b1 antibody
    • Transforming Growth Factor beta 1 antibody
    • Transforming growth factor beta 1a antibody
    • transforming growth factor beta-1 antibody
    • transforming growth factor, beta 1 antibody
    • Transforming Growth Factor-ß1 antibody
    see all

Images

  • Western blot of mouse 3T3 cell lysate with ab92486 at a concentration of 4 µg/ml.

  • ab92486 staining TGF beta 1 in Mouse intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 30 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in blocking buffer) for 24 hours at 4°C. An undiluted HRP-conjugated Human anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

  • ab92486 staining TGF beta 1 in Human retina membrane by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

    Tissue was fixed with paraformaldehyde and blocked with 10% serum for 30 minutes at 20°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/400 in PBS + 1% Triton X-100 + 2% goat serum) for 24 hours at 4°C. An HRP-conjugated Human polyclonal was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Pan J  et al. Pterostilbene, a bioactive component of blueberries, alleviates renal fibrosis in a severe mouse model of hyperuricemic nephropathy. Biomed Pharmacother 109:1802-1808 (2019). Read more (PubMed: 30551434) »
  • El-Agamy DS  et al. Pristimerin protects against doxorubicin-induced cardiotoxicity and fibrosis through modulation of Nrf2 and MAPK/NF-kB signaling pathways. Cancer Manag Res 11:47-61 (2019). Read more (PubMed: 30588110) »
See all 122 Publications for this product

Customer reviews and Q&As

1-10 of 18 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (Fibroblast)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (gel 12%)
Loading amount
30 µg
Specification
Fibroblast
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 21 2018

Application
Western blot
Sample
Pig Tissue lysate - whole (heart)
Gel Running Conditions
Reduced Denaturing (4-12% bis tris)
Loading amount
30 µg
Specification
heart
Blocking step
LIcor block buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Nov 30 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cynomolgus monkey Tissue sections (Spleen, Intestine, Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ventana CC2 (Low pH)
Permeabilization
No
Specification
Spleen, Intestine, Kidney
Blocking step
Background sniper as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 37°C
Fixative
Paraformaldehyde

Dr. Li Li

Verified customer

Submitted Oct 07 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Specification
Skin
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 27 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Chicken Cell (Aortic arches)
Permeabilization
No
Specification
Aortic arches
Blocking step
(agent) for 30 minute(s) · Concentration: 0.5% · Temperature: 4°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 20 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Mouse Tissue (Brain)
Specification
Brain

Dr. Kate Hawkins

Verified customer

Submitted Nov 23 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (skin)
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Acetone

Abcam user community

Verified customer

Submitted May 26 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Sample
Rabbit Tissue sections (intestinum)
Specification
intestinum
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 01 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Sample
Pig Tissue sections (liver)
Specification
liver
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated
Sample
Rat Tissue sections (brain)
Specification
brain
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 04 2013

1-10 of 18 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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