Recombinant Anti-TIL/TLR1 antibody [EPR2075] (ab68158)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2075] to TIL/TLR1
- Suitable for: WB, IP, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TIL/TLR1 antibody [EPR2075]
See all TIL/TLR1 primary antibodies -
Description
Rabbit monoclonal [EPR2075] to TIL/TLR1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human TIL/TLR1 aa 150-250 (extracellular). The exact sequence is proprietary.
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Positive control
- WB: Daudi and Ramos cell lysates. Flow Cyt: Daudi cells. IP: Ramos cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2075 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68158 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | ||
IP | ||
Flow Cyt |
Notes |
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IP: 1/40.
WB: 1/1000 - 1/2000. Detects a band of approximately 90 and 125 kDa (predicted molecular weight: 90 kDa).
Is unsuitable for ICC or IHC-P.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Participates in the innate immune response to microbial agents. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
Tissue specificity
Ubiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 12 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
Cellular localization
Cell membrane. Cytoplasmic vesicle > phagosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 7096 Human
- Omim: 601194 Human
- SwissProt: Q15399 Human
- Unigene: 621817 Human
- Unigene: 654532 Human
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Alternative names
- CD281 antibody
- CD281 antigen antibody
- KIAA0012 antibody
see all
Images
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All lanes : Anti-TIL/TLR1 antibody [EPR2075] (ab68158) at 1/1000 dilution
Lane 1 : Daudi cell lysate
Lane 2 : Ramos cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDa
Additional bands at: 125 kDa. We are unsure as to the identity of these extra bands. -
Flow cytometric analysis of TIL/TLR1 in Daudi cells using ab68158 at 1/60 dilution (pink) or a rabbit IgG (green).
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TIL/TLR1 was immunoprecipitated from 0.35 mg Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 µg with ab68158 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 µg
Lane 2: ab68158 IP in Ramos whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab68158 in Ramos whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab68158 has been referenced in 1 publication.
- Galoian K et al. Toll like receptors TLR1/2, TLR6 and MUC5B as binding interaction partners with cytostatic proline rich polypeptide 1 in human chondrosarcoma. Int J Oncol 52:139-154 (2018). PubMed: 29138803