• Product name
  • Description
    Rabbit polyclonal to TIMP1
  • Host species
  • Specificity
    This antibody has been used successfuly in mouse tissue in a publication, however our WB testing data shows non-reactivity to mouse and we can not guarantee reactivity with mouse.
  • Tested applications
    Suitable for: ELISA, WB, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from the internal region of human TIMP1.

  • Positive control
    • 293 cell extract, or rat brain lysate.



Our Abpromise guarantee covers the use of ab61224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
Flow Cyt Use at an assay dependent concentration. PubMed: 21133852

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration. PubMed: 21133852


  • Function
    Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
  • Sequence similarities
    Belongs to the protease inhibitor I35 (TIMP) family.
    Contains 1 NTR domain.
  • Post-translational
    The activity of TIMP1 is dependent on the presence of disulfide bonds.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Clgi antibody
    • Collagenase inhibitor antibody
    • Collagenase inhibitor, Human antibody
    • EPA antibody
    • EPO antibody
    • Erythroid Potentiating Activity antibody
    • Erythroid-potentiating activity antibody
    • Fibroblast collagenase inhibitor antibody
    • FLJ90373 antibody
    • HCI antibody
    • Human Collagenase Inhibitor antibody
    • Metalloproteinase inhibitor 1 antibody
    • Metalloproteinase inhibitor 1 precursor antibody
    • OTTHUMP00000023214 antibody
    • TIMP 1 antibody
    • TIMP antibody
    • TIMP metallopeptidase inhibitor 1 antibody
    • TIMP-1 antibody
    • Timp1 antibody
    • TIMP1 protein antibody
    • TIMP1_HUMAN antibody
    • Tissue Inhibitor of Metalloproteinase 1 antibody
    • Tissue inhibitor of metalloproteinases 1 antibody
    • Tissue inhibitor of metalloproteinases antibody
    • Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor antibody
    see all


  • All lanes : Anti-TIMP1 antibody (ab61224) at 1/500 dilution

    Lane 1 : extracts from 293 cells
    Lane 2 : extracts from 293 cells with the immunizing peptide

    Predicted band size: 23 kDa
    Observed band size: 23 kDa


This product has been referenced in:
  • Hu S  et al. Antifertility effectiveness of a novel copper-containing intrauterine device material and its influence on the endometrial environment in rats. Mater Sci Eng C Mater Biol Appl 89:444-455 (2018). Read more (PubMed: 29752117) »
  • Soler A  et al. Elevated 20-HETE in metabolic syndrome regulates arterial stiffness and systolic hypertension via MMP12 activation. J Mol Cell Cardiol 117:88-99 (2018). WB . Read more (PubMed: 29428638) »

See all 14 Publications for this product

Customer reviews and Q&As

Expiration date: March 9th, 2013
Value: ***

I am very pleased to hear you would like to accept our offer and test ab61224 in mouse. This code will give you *** off your next order before the expiration date. To r...

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Thank you for contacting Abcam.

Regarding ab1827 the full length protein was used as an immunogen.

Regarding ab61224, it is a peptide from the central region of the protein. The specific sequence is proprietary.
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Thank you for contacting us.

The immunogen for ab61224 correspond to thecentral region of human TIMP1. The full protein sequence can be found by clicking the link; http://www.uniprot.org/uniprot/P01033

The ab1827 is against the who...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caus...

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Thank you for taking the time to send me your protocols relating to this issue. I have been able to review them and agree that the problem here is the product. I would like to offer you free of charge replacements for ab1828 and ab61224. I may als...

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Thank you for contacting Abcam in regards to ab1828 and ab61224. I am sorry that you found that these products do not perform as well as expected in your applications.  I would appreciate it if you could provide me with protocol details of the appro...

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Thank you for taking time to complete our questionnaire. I am sorry to hear that antibodies ab16123, ab39184 and ab61224 are not providing satisfactory results. The details provided will enable us to investigate these cases and will provide us with ...

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Thank you for contacting us. We have indeed in our catalog 5 unconjugated anti-interferon alpha 1 antibodies tested and guaranteed to work in Western Blot and Human samples. Please note that, unfortunately, import restrictions make the ...

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