Anti-TLE4 antibody (ab64833)
Key features and details
- Rabbit polyclonal to TLE4
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-TLE4 antibody
See all TLE4 primary antibodies -
Description
Rabbit polyclonal to TLE4 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide corresponding to Human TLE4 (internal sequence).
Database link: Q04727 -
Positive control
- WB: Jurkat, 293, HuvEc cell; rat muscle lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64833 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa). |
Target
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Relevance
TLE4 (Transducin-like enhancer protein 4) is a member of the Groucho family of transcriptional corepressors and inhibits the transcriptional activation mediated by Pax5 and by beta-catenin and TCF family members in Wnt signaling. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 7091 Human
- Entrez Gene: 25565 Rat
- Omim: 605132 Human
- SwissProt: Q04727 Human
- SwissProt: Q07141 Rat
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Alternative names
- B lymphocyte gene 1 antibody
- E(sp1) homolog Drosophila antibody
- E(spI) antibody
see all
Images
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All lanes : Anti-TLE4 antibody (ab64833) at 1/500 dilution
Lane 1 : Jurkat cell extract at 30 µg
Lane 2 : 293 cell extract at 30 µg
Lane 3 : HuvEc cell extract at 30 µg
Lane 4 : HuvEc cell extract immunising peptide at 10 µg
Predicted band size: 84 kDa
Observed band size: 84 kDa -
Anti-TLE4 antibody (ab64833) at 1/1000 dilution + Rat muscle lysate
Predicted band size: 84 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab64833 has been referenced in 4 publications.
- Wakhloo D et al. Functional hypoxia drives neuroplasticity and neurogenesis via brain erythropoietin. Nat Commun 11:1313 (2020). PubMed: 32152318
- Patel D et al. The histone demethylase LSD1 regulates inner ear progenitor differentiation through interactions with Pax2 and the NuRD repressor complex. PLoS One 13:e0191689 (2018). PubMed: 29370269
- Bandyopadhyay S et al. Tle4 regulates epigenetic silencing of gamma interferon expression during effector T helper cell tolerance. Mol Cell Biol 34:233-45 (2014). PubMed: 24190972
- Wheat JC et al. The corepressor tle4 is a novel regulator of murine hematopoiesis and bone development. PLoS One 9:e105557 (2014). WB ; Mouse . PubMed: 25153823