Anti-TLR3 antibody (ab62566)
Key features and details
- Rabbit polyclonal to TLR3
- Suitable for: IHC-P, ICC, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TLR3 antibody
See all TLR3 primary antibodies -
Description
Rabbit polyclonal to TLR3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
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Positive control
- IHC-P: Human and mouse spleen tissue. WB: Daudi cell lysate and mouse speen tissue. ICC: Human spleen tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62566 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (2) |
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Also see abreview 35252. |
ICC |
Use a concentration of 1 µg/ml.
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WB | (6) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa).
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Notes |
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IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Also see abreview 35252. |
ICC
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa). |
Target
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Function
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
Tissue specificity
Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons. -
Involvement in disease
Defects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 22 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Contains 1 TIR domain. -
Domain
ds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18. -
Post-translational
modificationsHeavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding. -
Cellular localization
Endoplasmic reticulum membrane. Endosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 7098 Human
- Entrez Gene: 142980 Mouse
- Omim: 603029 Human
- SwissProt: O15455 Human
- SwissProt: Q99MB1 Mouse
- Unigene: 657724 Human
- Unigene: 33874 Mouse
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Alternative names
- CD283 antibody
- CD283 antigen antibody
- IIAE2 antibody
see all
Images
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Paraffin-embedded mouse spleen tissue stained for TLR3 using ab62566 at 2 µg/ml in immunohistochemical analysis. DAB detection. Counterstained with hematoxylin.
Antigen Retrieval (Microwave Method).
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Western blot analysis of TLR3 expression in mouse spleen tissue lysate with ab62566 at (A) 0.5 and (B) 1µg/ml.
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Lane 1 : Anti-TLR3 antibody (ab62566) at 1 µg/ml
Lane 2 : Anti-TLR3 antibody (ab62566) at 2 µg/ml
All lanes : Daudi (Human Burkitt's lymphoma cell line) cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 104 kDa
Observed band size: 104 kDa -
Paraffin-embedded human spleen tissue stained for TLR3 using ab62566 at 5 µg/ml in immunohistochemical analysis. DAB detection. Counterstained with hematoxylin.
Antigen Retrieval (Microwave Method).
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Immunocytochemical analysis of human spleen tissue labeling TLR3 using ab62566 at 20 µg/ml (green).
Antigen Retrieval (Microwave Method).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (41)
ab62566 has been referenced in 41 publications.
- Huang YH et al. Heat Shock Protein 60 Restricts Release of Mitochondrial dsRNA to Suppress Hepatic Inflammation and Ameliorate Non-Alcoholic Fatty Liver Disease in Mice. Int J Mol Sci 23:N/A (2022). PubMed: 35009003
- Dias BT et al. Toll-Like Receptor- and Protein Kinase R-Induced Type I Interferon Sustains Infection of Leishmania donovani in Macrophages. Front Immunol 13:801182 (2022). PubMed: 35154115
- Koike A et al. Statins attenuate antiviral IFN-β and ISG expression via inhibition of IRF3 and JAK/STAT signaling in poly(I:C)-treated hyperlipidemic mice and macrophages. FEBS J 288:4249-4266 (2021). PubMed: 33452755
- Nihon-Yanagi Y et al. Immunohistochemical Analysis of Toll-Like Receptors, MyD88, and TRIF in Human Papillary Thyroid Carcinoma and Anaplastic Thyroid Carcinoma. J Thyroid Res 2021:4226491 (2021). PubMed: 34306609
- Ernst EH et al. Distinct expression patterns of TLR transcripts in human oocytes and granulosa cells from primordial and primary follicles. J Reprod Immunol 140:103125 (2020). PubMed: 32454326