Anti-TLR4 antibody (ab13556)
Key features and details
- Rabbit polyclonal to TLR4
- Suitable for: WB, IHC-P, IHC-Fr, Flow Cyt
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-TLR4 antibody
See all TLR4 primary antibodies -
Description
Rabbit polyclonal to TLR4 -
Host species
Rabbit -
Specificity
TLR4 expression levels and cleavage or degradation products can vary between different cell and tissue samples. Customers have observed this variability in WB band size and our laboratory has confirmed this variability as well observing lower molecular weight cleavage and degradation products and in some samples a lack of the full length TLR4 band. The TLR4 cleavage and degradation products and potential lack of full length TLR4 are well documented in the literature, including PMID 16885150 and 22927440. We recommend running a positive control human intestine tissue lysate. We have obtained both positive and negative feedback from researchers using this antibody with rat samples (see Abreviews). Due to the inconsistency, we have removed rat as a guaranteed species and welcome any further feedback from researchers using this antibody. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P MouseWB MouseRecombinant fragment
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Immunogen
Synthetic peptide corresponding to Human TLR4 aa 420-435.
Sequence:GLEQLEHLDFQ HSNLK
Database link: O00206
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13556 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| IHC-P |
Mouse
|
| WB |
Mouse
Recombinant fragment
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (5) |
1/500.
Use primary at 1/500 dlution in 1xTBS. Incubate at 4C for 18 hours. Secondary should be incubated at 1 hour at room temperature. |
| IHC-P | (2) |
Use at an assay dependent concentration.
|
| IHC-Fr | (5) |
Use at an assay dependent concentration.
|
| Flow Cyt | (3) |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
1/500. Use primary at 1/500 dlution in 1xTBS. Incubate at 4C for 18 hours. Secondary should be incubated at 1 hour at room temperature. |
|
IHC-P
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific. -
Tissue specificity
Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells. -
Involvement in disease
Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 18 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
Domain
The TIR domain mediates interaction with NOX4. -
Post-translational
modificationsN-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 7099 Human
- Entrez Gene: 21898 Mouse
- Omim: 603030 Human
- SwissProt: O00206 Human
- SwissProt: Q9QUK6 Mouse
- Unigene: 174312 Human
- Unigene: 38049 Mouse
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Alternative names
- ARMD10 antibody
- CD284 antibody
- CD284 antigen antibody
see all
Images
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Western blot - Anti-TLR4 antibody (ab13556)Anti-TLR4 antibody (ab13556) at 1/1000 dilution + partial recombinant mouse TLR4 protein, 100 ng
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Western blot - Anti-TLR4 antibody (ab13556)Anti-TLR4 antibody (ab13556) at 1/500 dilution + TLR4 transfected Baculovirus-Insect whole cell lysate at 10 µg
Secondary
Anti-Rabbit HRP conjugate at 1/2000 dilution
Exposure time: 6 minutes -
Immunohistochemistry (Frozen sections) - Anti-TLR4 antibody (ab13556)ab13556 at a 1/100 dilution staining TLR4 in mouse spleen tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR4 antibody (ab13556)ab13556 at a 1/100 dilution staining TLR4 in mouse spleen tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
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Flow Cytometry - Anti-TLR4 antibody (ab13556)This image is courtesy of an Abreview submitted by Dr alexandre garinab13556 diluted 1/100 detecting TLR4 transfected mouse CHO cells by flow cytometry. The cells were prepared by treatment with collagenase and incubated with the primary antibody for 1 hour at 22°C. An Alexa Fluor® 488 goat anti-rabbit was used as the secondary antibody. Cells gated on live.
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Immunohistochemistry (Frozen sections) - Anti-TLR4 antibody (ab13556)ab13556 at 1/100000 dilution (12 hrs at 4degC) staining TLR4 in mouse colitis colon tissue section by Immunohistochemistry (Formalin-fixed tissue sections). A biotin Goat Anti-Rabbit secondary was used at 1/2000 for 1 hour at RT. Counterstain: Methyl Green at 200µL for 2 mins at RT. Localization: Inflammatory cells.
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Flow Cytometry - Anti-TLR4 antibody (ab13556)Flow cytometry analysis of THP-1 (Human monocytic leukemia cell line). Cells were fixed with 2% formaldehyde for 10 minutes at room temperature. ab13556 was used at 2 µg/106 cells for 60 minutes at 37°C (green). Goat Anti- Rabbit Dylight 488 was used as a secondary antibody at 1/200 dilution for 40 minutes at 37°C. Isotype control was Rabbit IgG under the same conditions (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (151)
ab13556 has been referenced in 151 publications.
- Shi CX et al. Sevoflurane attenuates brain damage through inhibiting autophagy and apoptosis in cerebral ischemia-reperfusion rats. Mol Med Rep 21:123-130 (2020). PubMed: 31746402
- Kang X et al. The TLR4/ERK/PD-L1 axis may contribute to NSCLC initiation. Int J Oncol 57:456-465 (2020). PubMed: 32468028
- Jing N et al. Exogenous activation of cannabinoid-2 receptor modulates TLR4/MMP9 expression in a spinal cord ischemia reperfusion rat model. J Neuroinflammation 17:101 (2020). PubMed: 32248810
- Tong W et al. Resveratrol inhibits LPS-induced inflammation through suppressing the signaling cascades of TLR4-NF-?B/MAPKs/IRF3. Exp Ther Med 19:1824-1834 (2020). PubMed: 32104238
- Yang H et al. Procyanidin Compound (PC) Suppresses Lipopolysaccharide-Induced Cervical Cancer Cell Proliferation Through Blocking the TLR4/NF-?B Pathway. Cancer Manag Res 12:497-509 (2020). PubMed: 32158256