Anti-TLR4 antibody (ab13556)
- Datasheet
- References (68)
- Protocols
Overview
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Product nameAnti-TLR4 antibody
See all TLR4 primary antibodies -
DescriptionRabbit polyclonal to TLR4
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Host speciesRabbit
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SpecificityTLR4 expression levels and cleavage or degradation products can vary between different cell and tissue samples. Customers have observed this variability in WB band size and our laboratory has confirmed this variability as well observing lower molecular weight cleavage and degradation products and in some samples a lack of the full length TLR4 band. The TLR4 cleavage and degradation products and potential lack of full length TLR4 are well documented in the literature, including PMID 16885150 and 22927440. We recommend running a positive control human intestine tissue lysate. We have obtained both positive and negative feedback from researchers using this antibody with rat samples (see Abreviews). Due to the inconsistency, we have removed rat as a guaranteed species and welcome any further feedback from researchers using this antibody.
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Tested applicationsSuitable for: IHC-P, WB, ELISA, ICC/IF, ICC, IHC-Fr, Flow Cytmore details
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Species reactivityReacts with: Mouse, Human
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Immunogen
Synthetic peptide corresponding to Human TLR4 aa 420-435.
Sequence:GLEQLEHLDFQ HSNLK
Database link: O00206 -
Positive control
- overexpressed recombinant protein
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
PurityProtein G purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Corresponding Antibody
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab13556 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50. | |
| WB | 1/500. Use primary at 1/500 dlution in 1xTBS. Incubate at 4C for 18 hours. Secondary should be incubated at 1 hour at room temperature. |
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| ELISA | Use at an assay dependent concentration. PubMed: 24952384 | |
| ICC/IF | Use at an assay dependent concentration. | |
| ICC | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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FunctionCooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
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Tissue specificityHighly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
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Involvement in diseaseGenetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
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Sequence similaritiesBelongs to the Toll-like receptor family.
Contains 18 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
DomainThe TIR domain mediates interaction with NOX4.
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Post-translational
modificationsN-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 7099 Human
- Entrez Gene: 21898 Mouse
- Omim: 603030 Human
- SwissProt: O00206 Human
- SwissProt: Q9QUK6 Mouse
- Unigene: 174312 Human
- Unigene: 38049 Mouse
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Alternative names
- ARMD10 antibody
- CD284 antibody
- CD284 antigen antibody
see all
Images
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Western blot - Anti-TLR4 antibody (ab13556)Anti-TLR4 antibody (ab13556) at 1/500 dilution + TLR4 transfected Baculovirus-Insect whole cell lysate at 10 µg
Secondary
Anti-Rabbit HRP conjugate at 1/2000 dilution
Exposure time: 6 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR4 antibody (ab13556)ab13556 staining TLR4 in human mouse spleen tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
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Immunocytochemistry - Anti-TLR4 antibody (ab13556)ab13556 positively staining formaldehyde fixed rat primary microglia cells (1/50), used in conjunction with goat anti rabbit Alex Fluor® 488 (1/250).
This image is courtesy of an Abreview submitted on 3 October 2005. We do not have any further information relating to this image. -
Flow Cytometry - Anti-TLR4 antibody (ab13556)This image is courtesy of an Abreview submitted by Dr alexandre garinab13556 diluted 1/100 detecting TLR4 transfected mouse CHO cells by flow cytometry. The cells were prepared by treatment with collagenase and incubated with the primary antibody for 1 hour at 22°C. An Alexa Fluor® 488 goat anti-rabbit was used as the secondary antibody. Cells gated on live.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR4 antibody (ab13556)ab13556 at 1/20000 dilution staining TLR4 in mouse colitis colon tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with two, 5 minutes incubation intervals in citrate buffer. An antibody amplifier™ was used for staining. A HRP-conjugated goat polyclonal to rabbit IgG was used as secondary at 1/10 dilution.
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Flow Cytometry - Anti-TLR4 antibody (ab13556)Flow cytometry analysis of Human monocytic Leukemia cells (THP-1). Cells were fixed with 2% Formaldehyde for 10 minutes at room temperature. Ab13556 was used at 2 µg/106 cells for 60 minutes at 37°C (green). Goat Anti- Rabbit Dylight 488 was used as a secondary antibody at 1:200 dilution for 40minutes at 37°C. Isotype control was Rabbit IgG under the same conditions (blue).
Protocols
References
This product has been referenced in:
- Göttle P et al. Rescuing the negative impact of human endogenous retrovirus envelope protein on oligodendroglial differentiation and myelination. Glia 67:160-170 (2019). Read more (PubMed: 30430656) »
- Yang WS et al. Ectodomain Shedding of RAGE and TLR4 as a Negative Feedback Regulation in High-Mobility Group Box 1-Activated Aortic Endothelial Cells. Cell Physiol Biochem 51:1632-1644 (2018). Read more (PubMed: 30497069) »
