Anti-TLR5 antibody [19D759.2] (ab13876)
Key features and details
- Mouse monoclonal [19D759.2] to TLR5
- Suitable for: IP, ICC, WB, Flow Cyt (Intra), ICC/IF, IHC-P, IHC-Fr
- Reacts with: Mouse, Human
- Isotype: IgG2a
Overview
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Product name
Anti-TLR5 antibody [19D759.2]
See all TLR5 primary antibodies -
Description
Mouse monoclonal [19D759.2] to TLR5 -
Host species
Mouse -
Specificity
This antibody recognizes an intracellular epitope in the cytoplasmic domain of TLR5. -
Tested applications
Suitable for: IP, ICC, WB, Flow Cyt (Intra), ICC/IF, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide: SSKDFTWVQN ALLKHLDTQY SDQNRFNLCF EERDFVPGEN RIANIQDAIW NSRKIVCLVS RHFLRDGWCL EAFSYAQGRC LSDLNSALIM VVVGSLSQY QL conjugated to KLH, corresponding to amino acids 700-800 of Human TLR5.
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Positive control
- Flow Cyt (Intra): Ramos cells WB: Ramos and Raw cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
19D759.2 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP | (2) |
Use at an assay dependent concentration.
|
ICC | (1) |
Use at an assay dependent concentration.
|
WB | (2) |
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 103 kDa.
|
Flow Cyt (Intra) |
Use 2µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody |
|
ICC/IF | (1) |
Use at an assay dependent concentration.
|
IHC-P | (2) |
Use at an assay dependent concentration.
Antigen retrieval with citrate buffer pH 6. |
IHC-Fr | (1) |
Use at an assay dependent concentration.
(see Miller et al). |
Notes |
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IP
Use at an assay dependent concentration. |
ICC
Use at an assay dependent concentration. |
WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 103 kDa. |
Flow Cyt (Intra)
Use 2µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Antigen retrieval with citrate buffer pH 6. |
IHC-Fr
Use at an assay dependent concentration. (see Miller et al). |
Target
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Function
Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
Tissue specificity
Highly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis. -
Involvement in disease
Systemic lupus erythematosus 1 -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 22 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
Post-translational
modificationsPhosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines.
Phosphorylated at Tyr-798 upon flagellin binding; required for signaling. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 7100 Human
- Entrez Gene: 53791 Mouse
- Omim: 603031 Human
- SwissProt: O60602 Human
- SwissProt: Q9JLF7 Mouse
- Unigene: 604542 Human
- Unigene: 116894 Mouse
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Alternative names
- FLJ10052 antibody
- MGC126430 antibody
- MGC126431 antibody
see all
Images
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Flow Cytometry (Intracellular) analysis of TLR5 in 106 Ramos cells using (A) 2µg of isotype control and (B) 2µg of ab13876
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All lanes : Anti-TLR5 antibody [19D759.2] (ab13876) at 2 µg/ml
Lane 1 : Ramos cell lysate
Lane 2 : Raw cell lysate
Predicted band size: 103 kDa
Western blot analysis of TLR5 in human Ramos cell lysate (A) and mouse Raw cell,lysate (B) using ab13876 at 2 g/mlµ
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab13876 has been referenced in 14 publications.
- Jiang H et al. M1 macrophage-derived exosomes and their key molecule lncRNA HOTTIP suppress head and neck squamous cell carcinoma progression by upregulating the TLR5/NF-κB pathway. Cell Death Dis 13:183 (2022). PubMed: 35210436
- Ruan G et al. Roseburia intestinalis and Its Metabolite Butyrate Inhibit Colitis and Upregulate TLR5 through the SP3 Signaling Pathway. Nutrients 14:N/A (2022). PubMed: 35893896
- Danielsen EM et al. Short-term tissue permeability actions of dextran sulfate sodium studied in a colon organ culture system. Tissue Barriers 8:1728165 (2020). PubMed: 32079482
- Ifuku M et al. Activation of Toll-like receptor 5 in microglia modulates their function and triggers neuronal injury. Acta Neuropathol Commun 8:159 (2020). PubMed: 32912327
- Sun M et al. The prognostic value of toll-like receptor5 and programmed cell death-ligand1 in patients with peripheral T-cell non-Hodgkin lymphoma. Leuk Lymphoma N/A:1-12 (2019). PubMed: 31017033