Recombinant Anti-TLR5 antibody [EPR10373] (ab168382)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10373] to TLR5
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TLR5 antibody [EPR10373]
See all TLR5 primary antibodies -
Description
Rabbit monoclonal [EPR10373] to TLR5 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human TLR5.
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Positive control
- HT29, A549, THP1 and HeLa cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10373 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168382 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 97 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 97 kDa. |
Target
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Function
Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
Tissue specificity
Highly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis. -
Involvement in disease
Systemic lupus erythematosus 1 -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 22 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
Post-translational
modificationsPhosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines.
Phosphorylated at Tyr-798 upon flagellin binding; required for signaling. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 7100 Human
- Entrez Gene: 53791 Mouse
- Entrez Gene: 289337 Rat
- Omim: 603031 Human
- SwissProt: O60602 Human
- SwissProt: Q9JLF7 Mouse
- Unigene: 604542 Human
- Unigene: 116894 Mouse
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Alternative names
- FLJ10052 antibody
- MGC126430 antibody
- MGC126431 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab168382 has been referenced in 4 publications.
- Shi D et al. TLR5: A prognostic and monitoring indicator for triple-negative breast cancer. Cell Death Dis 10:954 (2019). Mouse . PubMed: 31852883
- Lopetuso LR et al. Epithelial-specific Toll-like Receptor (TLR)5 Activation Mediates Barrier Dysfunction in Experimental Ileitis. Inflamm Bowel Dis 23:392-403 (2017). PubMed: 28146004
- Karlsen M et al. Expression of Toll-Like Receptors in Peripheral Blood Mononuclear Cells of Patients with Primary Sjögren's Syndrome. Scand J Immunol 85:220-226 (2017). PubMed: 27943374
- Menon BB et al. Suppression of Toll-like receptor-mediated innate immune responses at the ocular surface by the membrane-associated mucins MUC1 and MUC16. Mucosal Immunol 8:1000-8 (2015). PubMed: 25563498