Anti-TMPRSS5 antibody (ab111695)
Key features and details
- Rabbit polyclonal to TMPRSS5
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TMPRSS5 antibody
See all TMPRSS5 primary antibodies -
Description
Rabbit polyclonal to TMPRSS5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 175-402 of Human TMPRSS5 (AAI21804).
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Positive control
- MCF7 cells and whole cell lysate; U373 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab111695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 46 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 46 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/500. |
Target
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Function
May play a role in hearing. -
Tissue specificity
Brain-specific. Predominantly expressed in neurons, in their axons, and at the synapses of motoneurons in the spinal cord. -
Involvement in disease
Note=Defects in TMPRSS5 may be a cause of deafness. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 1 SRCR domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 80975 Human
- Entrez Gene: 80893 Mouse
- Entrez Gene: 266681 Rat
- Omim: 606751 Human
- SwissProt: Q9H3S3 Human
- SwissProt: Q9ER04 Mouse
- Unigene: 46720 Human
- Unigene: 72799 Mouse
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Alternative names
- MGC141886 antibody
- MGC148044 antibody
- OTTHUMP00000238209 antibody
see all
Images
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ab111695 at 1/500 dilution staining TMPRSS5 in Formalin-fixed, paraffin-embedded U373 xenograft tissue by Immunohistochemistry.
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Anti-TMPRSS5 antibody (ab111695) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg
Predicted band size: 46 kDa
10% SDS-PAGE. -
ab111695 at 1/200 dilution staining TMPRSS5 in methanol-fixed MCF7 cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab111695 has not yet been referenced specifically in any publications.