Anti-TNF alpha antibody (ab6671)
Rabbit polyclonal TNF alpha antibody. Validated in WB, ELISA, IHC, Neut, ICC/IF and tested in Mouse, Rat, Guinea pig, Human, Pig, Fish, Cynomolgus monkey. Cited in 230 publication(s).
- Datasheet
- References (289)
- Protocols
Overview
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Product nameAnti-TNF alpha antibody
See all TNF alpha primary antibodies -
DescriptionRabbit polyclonal to TNF alpha
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Host speciesRabbit
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Specificity
The immunogen used to raise this antibody shares 78% homology with mouse and rat TNFalpha protein. Some customers have successfully used ab6761 with mouse and rat samples, however we do not batch test in these species and therefore we cannot guarantee this product will consistently work with mouse and rat. We would recommend ab205587 or ab1793 as an alternative product for use with these species.
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Tested applicationsSuitable for: ELISA, IHC-P, IHC-Fr, Neutralising, WB, ICC/IF, IHC-FoFrmore details
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Species reactivityReacts with: Human, Cynomolgus monkey
Predicted to work with: Mouse, Rat, Dog, Pig, Monkey, Non human primates
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Immunogen
Recombinant full length protein corresponding to TNF alpha.
Database link: P01375 -
Positive control
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General notesEndotoxin content by LAL is <10 pg/ml.
Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).
See other anti-rabbit secondary antibodies that can be used with this antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
PurityIgG fraction
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Affibody® Molecule
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab6671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/200 - 1/1000. | |
| IHC-P | 1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| IHC-Fr | 1/100 - 1/200. | |
| Neutralising | 1/200. | |
| WB | 1/500 - 1/2000. Membrane Blocking is recommended with BSA not Milk for this product. Suitable for use as a positive control for Western blot against recombinant TNFalpha produced in E.Coli. |
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| ICC/IF | Use at an assay dependent concentration. PubMed: 19458984 | |
| IHC-FoFr | Use at an assay dependent concentration. |
Target
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FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
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Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
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Sequence similaritiesBelongs to the tumor necrosis factor family.
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Post-translational
modificationsThe soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. -
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 7124 Human
- Entrez Gene: 21926 Mouse
- Entrez Gene: 24835 Rat
- Omim: 191160 Human
- SwissProt: P79337 Cynomolgus monkey
- SwissProt: P51742 Dog
- SwissProt: P01375 Human
- SwissProt: P06804 Mouse
see all -
Alternative names
- APC1 antibody
- APC1 protein antibody
- Cachectin antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody (ab6671)Image is courtesy of an anonymous Abreviewab6671 staining TNF alpha in Human testis & colon CA tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% H2O2 for 10 minutes at 25°C; antigen retrieval was by heat mediation using EDTA, pH9, 100C, 20 min. Samples were incubated with primary antibody (1/75) for 20 minutes at 25°C. An undiluted HRP polymer-conjugated Goat anti-mouse anti-rabbit polyclonal was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody (ab6671)Image courtesy of Matthew Williamsab6671 staining TNF alpha in Guinea pig Lung and heart cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formalin and blocked with 1% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 1% BSA in PBST) for 18 hours at 4°C. An Alexa Fluor® 555-conjugated Donkey anti-rabbit IgG (H+L) polyclonal (1/800) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody (ab6671)This image is courtesy of an Abreview submitted by Jing Maab6671 staining TNF alpha in Cynomolgus Monkey dendritic cells/macrophages from inlamed skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 20 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer, pH6.0. Samples were incubated with primary antibody (1/100) for 30 minutes at room temperature. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/2000) was used as the secondary antibody.
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Western blot - Anti-TNF alpha antibody (ab6671)Anti-TNF alpha antibody (ab6671) at 1/1000 dilution + Recombinant TNF-alpha protein
Secondary
Goat Anti-Rabbit Dylight® 649 Conjugate at 1/20000 dilution
Additional bands at: 16 kDa (possible cleavage fragment)Western Blot analysis, labelling TNF-alpha with ab6671 at 1/1000. Blocking was with 1% BSA in TBS-T incubated for 30 minutes at room temperature. The Cleavage Fragment is related to the soluble isoform 77 - 223.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody (ab6671)This image is courtesy of an anonymous Abreviewab6671 staining human artery tissue sections by IHC-P. Sections were fixed in formaldehyde and subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0) prior to blocking with 1.5% serum for 10 minutes. The primary antibody was diluted 1/100 and incubated with the sample for 24 hours at 4°C. An HRP-conjugated goat anti-rabbit antibody was used as the secondary.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody (ab6671)This image is courtesy of an Abreview submitted by Nicole Schechterab6671 staining TNF alpha from human colon by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were formaldehyde fixed prior to blocking in 10% serum for 2 hours at 21°C. The primary antibody was diluted 1/100 and incubated with the sample for 2 hours at 21°C. Alexa fluor® 680 goat polyclonal, diluted 1/5000, was used as the secondary. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody (ab6671)This image is courtesy of an Abreview submitted by Paolo RonzaImmunohistochemical analysis of formalin-fixed, paraffin embedded fish tissue, staining TNF alpha with ab6671.
Tissue was fixed with Bouin's fixative; antigen retrieval was by heat mediation in a citrate buffer (pH 6). Samples were incubated with primary antibody (1/600 in diluent) for 2 hours. An HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.
Protocols
References
This product has been referenced in:
- Dormer NH et al. Evaluation of a transtympanic delivery system in Mus musculus for extended release steroids. Eur J Pharm Sci 126:3-10 (2019). Read more (PubMed: 29329746) »
- Sayyaf Dezfuli B et al. Description of epithelial granular cell in catshark spiral intestine: Immunohistochemistry and ultrastructure. J Morphol 280:205-213 (2019). Read more (PubMed: 30589112) »
