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  1. Link

    tnf-alpha-antibody-ab6671.pdf

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Immunology Innate Immunity Cytokines TNF Superfamily
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Anti-TNF alpha antibody (ab6671)

  • Datasheet
Reviews (34)Q&A (24)References (641)

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Western blot - Anti-TNF alpha antibody (ab6671)

    Key features and details

    • Rabbit polyclonal to TNF alpha
    • Suitable for: ELISA, IHC-P, ICC/IF, WB, Neutralising
    • Reacts with: Human, Cynomolgus monkey, Recombinant fragment
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-TNF alpha antibody [EPR20972] (ab215188)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-TNF alpha antibody
      See all TNF alpha primary antibodies
    • Description

      Rabbit polyclonal to TNF alpha
    • Host species

      Rabbit
    • Specificity

      The immunogen used to raise this antibody shares 78% homology with mouse and rat TNF alpha protein. Some customers have successfully used ab6671 with mouse and rat samples, however we do not batch test in these species and therefore we cannot guarantee this product will consistently work with mouse and rat.  We would recommend ab205587 or ab1793 as an alternative product for use with these species.

      The antibody does not recognize human TNF beta (lymphotoxin).

    • Tested applications

      Suitable for: ELISA, IHC-P, ICC/IF, WB, Neutralisingmore details
    • Species reactivity

      Reacts with: Human, Cynomolgus monkey, Recombinant fragment
      Predicted to work with: Mouse, Rat, Dog, Pig, Monkey, Non human primates
    • Immunogen

      Recombinant full length protein corresponding to Human TNF alpha.
      Database link: P01375

    • Positive control

      • Purchase matching WB positive control:Recombinant Human TNF alpha protein
      • WB: Recombinant TNF-alpha protein.
    • General notes

      Endotoxin content by LAL is <10 pg/ml.

      Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).

      See other anti-rabbit secondary antibodies that can be used with this antibody.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
    • Concentration information loading...
    • Purity

      IgG fraction
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Immunology
      • Innate Immunity
      • Cytokines
      • TNF Superfamily
      • Signal Transduction
      • Growth Factors/Hormones
      • TNF
      • Microbiology
      • Organism
      • Virus
      • RNA Virus
      • ssRNA positive strand virus
      • SARS Coronavirus
      • Cancer
      • Growth factors
      • TNF
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Inflammatory mediators
      • Metabolism
      • Types of disease
      • Metabolic disorders
      • Neuroscience
      • Development
      • Neuroscience
      • Diseases
      • Neuroscience
      • Processes

    Associated products

    • Affibody® Molecule

      • Anti-TNF alpha Affibody® Molecule (ab31908)
      • Anti-TNF alpha Affibody® Molecule (Agarose) (ab31909)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human TNF alpha protein (Active) (ab259410)
      • Recombinant mouse TNF alpha protein (Active) (ab259411)
    • Related Products

      • Rat TNF alpha ELISA Kit (ab100785)
      • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
      • Human TNF alpha ELISA Kit (ab181421)
      • Mouse TNF alpha ELISA Kit (ab208348)
      • Mouse TNF alpha Matched Antibody Pair Kit (ab212073)
      • Human TNF alpha Matched Antibody Pair Kit (ab213467)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab6671 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA
    Use at an assay dependent concentration.
    IHC-P (19)
    Use at an assay dependent concentration.
    ICC/IF (1)
    Use at an assay dependent concentration. PubMed: 19458984
    WB (10)
    1/500 - 1/2000. Predicted molecular weight: 25.6 kDa.

    Membrane Blocking is recommended with BSA not Milk for this product.

    Suitable for use as a positive control for Western blot against recombinant TNFalpha produced in E.Coli. 

    Neutralising
    Use at an assay dependent concentration.
    IF
    Use at an assay dependent concentration.
    Notes
    ELISA
    Use at an assay dependent concentration.
    IHC-P
    Use at an assay dependent concentration.
    ICC/IF
    Use at an assay dependent concentration. PubMed: 19458984
    WB
    1/500 - 1/2000. Predicted molecular weight: 25.6 kDa.

    Membrane Blocking is recommended with BSA not Milk for this product.

    Suitable for use as a positive control for Western blot against recombinant TNFalpha produced in E.Coli. 

    Neutralising
    Use at an assay dependent concentration.
    IF
    Use at an assay dependent concentration.

    Target

    • Function

      Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
    • Involvement in disease

      Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
    • Sequence similarities

      Belongs to the tumor necrosis factor family.
    • Post-translational
      modifications

      The soluble form derives from the membrane form by proteolytic processing.
      The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
      O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
    • Cellular localization

      Secreted and Cell membrane.
    • Target information above from: UniProt accession P01375 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7124 Human
      • Entrez Gene: 21926 Mouse
      • Entrez Gene: 24835 Rat
      • Omim: 191160 Human
      • SwissProt: P79337 Cynomolgus monkey
      • SwissProt: P51742 Dog
      • SwissProt: P01375 Human
      • SwissProt: P06804 Mouse
      • SwissProt: P23563 Pig
      • SwissProt: P16599 Rat
      • Unigene: 241570 Human
      • Unigene: 1293 Mouse
      • Unigene: 2275 Rat
      see all
    • Alternative names

      • APC1 antibody
      • APC1 protein antibody
      • Cachectin antibody
      • DIF antibody
      • Differentiation inducing factor antibody
      • Macrophage cytotoxic factor antibody
      • Tnf antibody
      • TNF superfamily member 2 antibody
      • TNF superfamily, member 2 antibody
      • TNF, macrophage derived antibody
      • TNF, monocyte derived antibody
      • TNF-a antibody
      • TNF-alpha antibody
      • TNFA antibody
      • TNFA_HUMAN antibody
      • TNFSF2 antibody
      • Tumor necrosis factor (TNF superfamily member 2) antibody
      • Tumor necrosis factor alpha antibody
      • Tumor necrosis factor antibody
      • Tumor necrosis factor ligand superfamily member 2 antibody
      • Tumor Necrosis Factor, Membrane Form antibody
      • Tumor necrosis factor, soluble form antibody
      see all

    Images

    • Western blot - Anti-TNF alpha antibody (ab6671)
      Western blot - Anti-TNF alpha antibody (ab6671)
      Anti-TNF alpha antibody (ab6671) at 1/1000 dilution + Recombinant TNF alpha protein

      Secondary
      Goat Anti-Rabbit Dylight® 649 Conjugate at 1/20000 dilution

      Predicted band size: 25.6 kDa
      Additional bands at: 16 kDa (possible cleavage fragment)



      Western Blot analysis, labeling TNF alpha with ab6671 at 1/1000. Blocking was with 1% BSA in TBS-T incubated for 30 minutes at room temperature. The cleavage fragment is related to the soluble isoform 77 - 223. 

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (641)

    Publishing research using ab6671? Please let us know so that we can cite the reference in this datasheet.

    ab6671 has been referenced in 641 publications.

    • Pang N  et al. Transcranial Ultrasound Stimulation of Hypothalamus in Aging Mice. IEEE Trans Ultrason Ferroelectr Freq Control 68:29-37 (2021). PubMed: 31985418
    • Kostakoglu U  et al. The Protective Effects of Perindopril Against Acute Kidney Damage Caused by Septic Shock. Inflammation 44:148-159 (2021). PubMed: 32803666
    • Chen Q  et al. Shenyan Kangfu tablet alleviates diabetic kidney disease through attenuating inflammation and modulating the gut microbiota. J Nat Med 75:84-98 (2021). PubMed: 32997272
    • Saadati H  et al. The Neuroprotective Effect of Mesna on Cisplatin-Induced Neurotoxicity: Behavioral, Electrophysiological, and Molecular Studies. Neurotox Res 39:826-840 (2021). PubMed: 33216283
    • Wang Q  et al. Association of miRNA-145 with the occurrence and prognosis of hydrosalpinx-induced defective endometrial receptivity. Bosn J Basic Med Sci 21:81-92 (2021). PubMed: 32343942
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 58 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TNF alpha antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: high Target retrival solution
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5µg/mL · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    FRAU. Ines Kleiber-Schaaf

    Verified customer

    Submitted Nov 04 2020

    Western blot abreview for Anti-TNF alpha antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Glioblastoma cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Treatment
    4mg/ml bevacizumab for 48hrs
    Specification
    Glioblastoma cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 09 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-TNF alpha antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (bone)
    Permeabilization
    No
    Specification
    bone
    Blocking step
    Vetor Blocker as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 28 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TNF alpha antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Derm)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Vetor Unmasking solution
    Permeabilization
    Yes - 0.05% triton-x100
    Specification
    Derm
    Blocking step
    Vetor Blocker as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 28 2019

    Western blot abreview for Anti-TNF alpha antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Lung)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    50 µg
    Specification
    Lung
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 07 2019

    Western blot abreview for Anti-TNF alpha antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Goat Tissue lysate - other (Goat MSCs and Goat Nucleus pulposus cells pellets)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (10%)
    Loading amount
    20 µg
    Treatment
    1 ug/mL Lipopolysaccharide induced 3 days
    Specification
    Goat MSCs and Goat Nucleus pulposus cells pellets
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 26°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Chenghao Zhang

    Verified customer

    Submitted Sep 07 2018

    Western blot abreview for Anti-TNF alpha antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (OV90 cells)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris Glycin gel, semi dry transfer)
    Loading amount
    50 µg
    Specification
    OV90 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 18 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TNF alpha antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (vessel)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: H2O2
    Permeabilization
    No
    Specification
    vessel
    Blocking step
    dako antibody diluent with background reducing components as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 30 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TNF alpha antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Neuroblastoma)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 8.5
    Permeabilization
    No
    Specification
    Neuroblastoma
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Allan Wang

    Verified customer

    Submitted Apr 18 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-TNF alpha antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (molar injury)
    Permeabilization
    Yes - Triton X
    Specification
    molar injury
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Tina Yuan

    Verified customer

    Submitted Jan 25 2018

    1-10 of 58 Abreviews or Q&A

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