Anti-TPC2 antibody (ab119915)
Key features and details
- Rabbit polyclonal to TPC2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TPC2 antibody -
Description
Rabbit polyclonal to TPC2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human TPC2 (N terminal).
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Positive control
- MCF7 cell lysate, Human fetal small intestine tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituent: 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab119915 is purified by a peptide affinity column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 98 kDa (predicted molecular weight: 85 kDa).
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IHC-P |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 98 kDa (predicted molecular weight: 85 kDa). |
IHC-P
1/100 - 1/500. |
Target
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Function
Nicotinic acid adenine dinucleotide phosphate (NAADP) receptor that may function as one of the major voltage-gated Ca(2+) channels (VDCC) across the lysosomal membrane. May be involved in smooth muscle contraction. -
Tissue specificity
Widely expressed. Expressed at high level in liver and kidney. -
Sequence similarities
Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. Two pore calcium channel subfamily. -
Domain
Each of the two internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Lysosome membrane. Only the acidic lysosomal fraction is sensitive to NAADP. - Information by UniProt
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Database links
- Entrez Gene: 219931 Human
- Entrez Gene: 233979 Mouse
- Entrez Gene: 309139 Rat
- Omim: 612163 Human
- SwissProt: Q8NHX9 Human
- SwissProt: Q8BWC0 Mouse
- Unigene: 131851 Human
- Unigene: 102235 Mouse
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Alternative names
- SHEP10 antibody
- TPC2 antibody
- TPC2_HUMAN antibody
see all
Images
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Anti-TPC2 antibody (ab119915) at 1/500 dilution + MCF7 cell lysate
Predicted band size: 85 kDaThe amount of protein per lane: 30ug
PVDF membrane
Blocking solution: 5% (W/V) skim milk, with 1 X TBST buffer
Shake membrane at room temperature with ab119915, 1/500, for 30 minutes, then transfer to 4°C overnight. -
ab119915 at 1/100 dilution staining TPC2 in Formalin-fixed, Paraffin-embedded Human fetal small intestine tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab119915 has been referenced in 6 publications.
- Nabar NR et al. LRRK2 is required for CD38-mediated NAADP-Ca2+ signaling and the downstream activation of TFEB (transcription factor EB) in immune cells. Autophagy 18:204-222 (2022). PubMed: 34313548
- Faris P et al. Nicotinic Acid Adenine Dinucleotide Phosphate Induces Intracellular Ca2+ Signalling and Stimulates Proliferation in Human Cardiac Mesenchymal Stromal Cells. Front Cell Dev Biol 10:874043 (2022). PubMed: 35392169
- Hu W et al. NAADP-induced intracellular calcium ion is mediated by the TPCs (two-pore channels) in hypoxia-induced pulmonary arterial hypertension. J Cell Mol Med 25:7485-7499 (2021). PubMed: 34263977
- Khan N et al. Two-pore channels regulate Tat endolysosome escape and Tat-mediated HIV-1 LTR transactivation. FASEB J 34:4147-4162 (2020). PubMed: 31950548
- He T et al. Inhibition of two-pore channels in antigen-presenting cells promotes the expansion of TNFR2-expressing CD4+Foxp3+ regulatory T cells. Sci Adv 6:N/A (2020). PubMed: 32998896
- Lin JR et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. Elife 7:N/A (2018). PubMed: 29993362