Anti-TRA2A antibody (ab169052)
Key features and details
- Mouse polyclonal to TRA2A
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-TRA2A antibody
See all TRA2A primary antibodies -
Description
Mouse polyclonal to TRA2A -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein within Human TRA2A aa 1-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
-
Positive control
- HepG2 whole cell lysate (ab7900), Raw 267.4 cell lysate, NIH 3T3 cell lysate, TRA2A transfected 293T cell lysate; HeLa cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 99% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169052 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa.
|
|
ICC/IF |
Use a concentration of 10 µg/ml.
|
Notes |
---|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
-
Function
Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing. -
Sequence similarities
Belongs to the splicing factor SR family.
Contains 1 RRM (RNA recognition motif) domain. -
Post-translational
modificationsPhosphorylated in the RS domains. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 29896 Human
- Entrez Gene: 101214 Mouse
- Omim: 602718 Human
- SwissProt: Q13595 Human
- SwissProt: Q6PFR5 Mouse
- Unigene: 445652 Human
- Unigene: 196598 Mouse
-
Alternative names
- AWMS1 antibody
- HSU53209 antibody
- htra-2 alpha antibody
see all
Images
-
Anti-TRA2A antibody (ab169052) at 1 µg/ml + HepG2 cell lysate at 50 µg
Secondary
Goat anti-mouse HRP conjugated antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 32 kDa -
Anti-TRA2A antibody (ab169052) at 1 µg/ml + Raw 267.4 cell lysate at 50 µg
Secondary
Goat anti-mouse HRP conjugated antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 32 kDa -
Anti-TRA2A antibody (ab169052) at 1 µg/ml + NIH 3T3 cell lysate at 50 µg
Secondary
Goat anti-mouse HRP conjugated antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 32 kDa -
All lanes : Anti-TRA2A antibody (ab169052) at 1 µg/ml
Lane 1 : TRA2A transfected 293T cell lysate
Lane 2 : non 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat anti-mouse HRP conjugated antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 32 kDa -
Immunofluorescent analysis of HeLa cells labeling TRA2A with ab169052 at 10 µg/ml.
Protocols
Datasheets and documents
-
Datasheet download
References (0)
ab169052 has not yet been referenced specifically in any publications.