Anti-TRAF4 antibody (ab88612)
Key features and details
- Mouse polyclonal to TRAF4
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRAF4 antibody
See all TRAF4 primary antibodies -
Description
Mouse polyclonal to TRAF4 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human TRAF4. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- WB: human pancreas tissue lysate, TRAF4 transfected 293T cell lysate ICC/IF: HeLa cell
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Adapter protein and signal transducer that links members of the tumor necrosis factor receptor (TNFR) family to different signaling pathways. Plays a role in the activation of NF-kappa-B and JNK, and in the regulation of cell survival and apoptosis. Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for normal skeleton development, and for normal development of the respiratory tract (By similarity). Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions. -
Tissue specificity
Expressed in epithelial cells of thymus, dendritic cells of lymph node, and in the basal cell layer of epithelia such as epidermis, nasopharynx, respiratory tract, salivary gland, and esophagus. -
Sequence similarities
Belongs to the TNF receptor-associated factor family. B subfamily.
Contains 1 MATH domain.
Contains 1 RING-type zinc finger.
Contains 3 TRAF-type zinc fingers. -
Domain
The coiled coil domain mediates homo- and hetero-oligomerization.
The MATH/TRAF domain binds to receptor cytoplasmic domains. -
Post-translational
modificationsPolyubiquitinated, leading to its proteasomal degradation. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasm > perinuclear region. Cell junction > tight junction. Cell membrane. Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 9618 Human
- Omim: 602464 Human
- SwissProt: Q9BUZ4 Human
- Unigene: 8375 Human
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Alternative names
- CART 1 antibody
- CART1 antibody
- cysteine rich domain associated with ring and TRAF domain antibody
see all
Images
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Anti-TRAF4 antibody (ab88612) at 1 µg/ml + human pancreas tissue lysate at 50 µg
Predicted band size: 54 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted? -
All lanes : Anti-TRAF4 antibody (ab88612) at 1 µg/ml
Lane 1 : TRAF4 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 54 kDa
Observed band size: 54 kDa -
ab88612, at 10 µg/ml, staining TRAF4 on HeLa cell by Immunofluorescence.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab88612 has been referenced in 2 publications.
- Cen S et al. TRAF4 acts as a fate checkpoint to regulate the adipogenic differentiation of MSCs by activating PKM2. EBioMedicine 54:102722 (2020). PubMed: 32268273
- Li J et al. TRAF4 positively regulates the osteogenic differentiation of mesenchymal stem cells by acting as an E3 ubiquitin ligase to degrade Smurf2. Cell Death Differ 26:2652-2666 (2019). PubMed: 31076633