Anti-TRIM22 antibody (ab68071)
Key features and details
- Mouse polyclonal to TRIM22
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRIM22 antibody
See all TRIM22 primary antibodies -
Description
Mouse polyclonal to TRIM22 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human TRIM22 aa 1-500. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- Trim22 transfected mammalian cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa). |
Target
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Relevance
Trim22 is an interferon inducible protein that is preferentially expressed in cells of the haematopoietic system. Trim22 has been shown to be a p53 target gene. It also has an activation stage specific role connected to the paracrine crosstalk during T lymphocyte activation. It is strongly expressed in peripheral blood leukocytes, spleen, thymus, and ovary; expressed at basal levels in other tissues. There are two named isoforms. -
Cellular localization
Cytoplasm. Nucleus. Nucleus speckle. Nucleus; Cajal body. Note: Localizes predominanltly into the nucleus, found in cytoplasm to some extent. Forms distinct nuclear bodies that undergo dynamic changes during cell cycle progression. Nuclear bodies start to form in the early G0/G1 phase but become speckle-like in the S-phase and completely dispersed in mitosis. 35% of TRIM22 nuclear bodies overlap or are found adjacent to Cajal bodies. -
Database links
- Entrez Gene: 10346 Human
- Omim: 606559 Human
- SwissProt: Q8IYM9 Human
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Alternative names
- 50 kDa stimulated trans acting factor antibody
- GPSTAF50 antibody
- RING finger protein 94 antibody
see all
Images
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All lanes : Anti-TRIM22 antibody (ab68071) at 1/500 dilution
Lane 1 : Trim22 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 57 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (2)
ab68071 has been referenced in 2 publications.
- Chen J et al. MicroRNA-376b-3p Promotes Porcine Reproductive and Respiratory Syndrome Virus Replication by Targeting Viral Restriction Factor TRIM22. J Virol 96:e0159721 (2022). PubMed: 34757838
- Ye B & Lu Z Role of TRIM22 in ulcerative colitis and its underlying mechanisms. Mol Med Rep 26:N/A (2022). PubMed: 35674157