Product nameAnti-TRIM24 antibody
See all TRIM24 primary antibodies
DescriptionRabbit polyclonal to TRIM24
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide within Human TRIM24 aa 700-750. The exact sequence is proprietary.
Database link: NP_056989.2
- Whole cell lysates from HeLa, 293T and NIH3T3 cells. This antibody gave a positive result in IHC in the following FFPE tissue: Human colon adenocarcinoma
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 116 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionTranscriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes.
PathwayProtein modification; protein ubiquitination.
Involvement in diseaseDefects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TRIM24/TIF1 is found in thyroid papillary carcinomas. Translocation t(7;10)(q32;q11) with RET. The translocation generates the TRIM24/RET (PTC6) oncogene.
Sequence similaritiesContains 2 B box-type zinc fingers.
Contains 1 bromo domain.
Contains 1 PHD-type zinc finger.
Contains 1 RING-type zinc finger.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Cytoplasm. Colocalizes with sites of active transcription. Detected both in nucleus and cytoplasm in some breast cancer samples. Predominantly nuclear.
- Information by UniProt
- E3 ubiquitin protein ligase TRIM24 antibody
- E3 ubiquitin-protein ligase Trim24 antibody
- hTIF1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling TRIM24 with ab70560 at 1/1000 (0.2µg/ml). Detection: DAB.
IHC image of TRIM24 staining in Human colon adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70560, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-TRIM24 antibody (ab70560) at 0.04 µg/ml
Lane 1 : HeLa whole cell
lysate at 50 µg
Lane 2 : HeLa whole cell
lysate at 15 µg
Lane 3 : HeLa whole cell
lysate at 5 µg
Lane 4 : 293T whole cell
lysate at 50 µg
Lane 5 : NIH3T3whole cell
lysate at 50 µg
Predicted band size: 116 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
This product has been referenced in:
- Fyfe JJ et al. Enhanced skeletal muscle ribosome biogenesis, yet attenuated mTORC1 and ribosome biogenesis-related signalling, following short-term concurrent versus single-mode resistance training. Sci Rep 8:560 (2018). Read more (PubMed: 29330460) »
- Fang Z et al. TRIM24 promotes the aggression of gastric cancer via the Wnt/ß-catenin signaling pathway. Oncol Lett 13:1797-1806 (2017). WB, IHC-P ; Human . Read more (PubMed: 28454326) »