Overview

  • Product name

  • Description

    Rabbit polyclonal to TRIM24
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human TRIM24 aa 700-750.
    Database link: NP_056989.2

  • Positive control

    • Human: breast carcinoma, metastatic lymph node, ovarian carcinoma, prostate carcinoma, testicular seminoma. Mouse: squamous cell carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab84381 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/500. Epitope exposure with citrate buffer will enhance staining.
    Note: expected to work on frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes.
    • Pathway

      Protein modification; protein ubiquitination.
    • Involvement in disease

      Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TRIM24/TIF1 is found in thyroid papillary carcinomas. Translocation t(7;10)(q32;q11) with RET. The translocation generates the TRIM24/RET (PTC6) oncogene.
    • Sequence similarities

      Contains 2 B box-type zinc fingers.
      Contains 1 bromo domain.
      Contains 1 PHD-type zinc finger.
      Contains 1 RING-type zinc finger.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
      Sumoylated.
    • Cellular localization

      Nucleus. Cytoplasm. Colocalizes with sites of active transcription. Detected both in nucleus and cytoplasm in some breast cancer samples. Predominantly nuclear.
    • Information by UniProt
    • Database links

    • Alternative names

      • E3 ubiquitin protein ligase TRIM24 antibody
      • E3 ubiquitin-protein ligase Trim24 antibody
      • hTIF1 antibody
      • PTC6 antibody
      • RING finger protein 82 antibody
      • RNF82 antibody
      • TF1A antibody
      • TIF1 alpha antibody
      • TIF1 antibody
      • TIF1-alpha antibody
      • TIF1A antibody
      • TIF1A_HUMAN antibody
      • TIF1ALPHA antibody
      • Transcription intermediary factor 1-alpha antibody
      • Transcriptional intermediary factor 1 alpha antibody
      • Transcriptional intermediary factor 1 antibody
      • Trim24 antibody
      • Tripartite motif containing 24 antibody
      • Tripartite motif-containing protein 24 antibody
      see all

    Images

    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human breast tumor tissue using 1/250 ab84381, followed by DAB staining.
    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Mouse squamous cell carcinoma tissue using 1/250 ab84381, followed by DAB staining.

    References

    This product has been referenced in:

    • Kim Y  et al. Anti-TIF1? antibody and the expression of TIF1? in idiopathic inflammatory myopathies. Int J Rheum Dis N/A:N/A (2018). Read more (PubMed: 30398003) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Answer

    Thank you for contacting us.
    We have 3 antibodies to TIF1 alpha: ab70560, ab57994 and ab84381.
    Two of them have been tested in mouse (ab70560 and ab84381), and ab84381 was already tested and would be guaranteed for mouse and human paraffin-embedded IHC samples (IHC-P).
    What type of IHC do you have in mind?
    We guarantee all our products for tested species and applications as listed on the datasheet. The guarantee is valid for 6 months after a purchase. For more details about our Abpromise guarantee please check this link: https://www.abcam.com/abpromise.
    For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
    The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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