Anti-TSEN34 antibody (ab68868)
Key features and details
- Mouse polyclonal to TSEN34
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TSEN34 antibody
See all TSEN34 primary antibodies -
Description
Mouse polyclonal to TSEN34 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human TSEN34. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- TSEN34 transfected 293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituents: 0.2% Monobasic dihydrogen potassium phosphate, 0.6% Dibasic monohydrogen sodium phosphate, 8% Sodium chloride, 0.2% Potassium chloride, 91% Water -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68868 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa). |
Target
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Function
Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. -
Involvement in disease
Pontocerebellar hypoplasia 2C -
Sequence similarities
Belongs to the tRNA-intron endonuclease family. -
Cellular localization
Nucleus. Nucleus, nucleolus. May be transiently localized in the nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 79042 Human
- Omim: 608754 Human
- SwissProt: Q9BSV6 Human
- Unigene: 15580 Human
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Alternative names
- HsSen34 antibody
- LENG 5 antibody
- LENG5 antibody
see all
Images
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All lanes : Anti-TSEN34 antibody (ab68868) at 1/500 dilution
Lane 1 : TSEN34 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 43 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (1)
ab68868 has been referenced in 1 publication.
- Breuss MW et al. Autosomal-Recessive Mutations in the tRNA Splicing Endonuclease Subunit TSEN15 Cause Pontocerebellar Hypoplasia and Progressive Microcephaly. Am J Hum Genet 99:228-35 (2016). PubMed: 27392077