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RecombinantRabMAb

Recombinant Anti-TSG101 antibody [EPR7130(B)] (ab125011)

  • Datasheet
  • SDS
Reviews (8) Submit a question References (99)

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Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
  • Immunocytochemistry/ Immunofluorescence - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
  • Flow Cytometry - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
  • OI-RD Scanning - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
  • Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
  • Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
  • Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
  • Immunocytochemistry/ Immunofluorescence - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
  • Flow Cytometry - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
  • Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
  • Flow Cytometry - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
  • Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7130(B)] to TSG101
  • Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-TSG101 antibody [EPR7130(B)]
    See all TSG101 primary antibodies
  • Description

    Rabbit monoclonal [EPR7130(B)] to TSG101
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human TSG101 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • WB: K562, Jurkat, and SH-SY5Y cell lysates. Human brain lysate; mouse and rat heart tissue lysates. IHC-P: Human kidney tissue. ICC: HEK-293 cells. Flow Cyt: HeLa and K562 cells.
  • General notes

      

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 4.00 x 10 -12 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7130(B)
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Other
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • Other
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Differentiation
    • Cancer
    • Cell cycle
    • Cell differentiation
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • Other

Associated products

  • Alternative Versions

    • Anti-TSG101 antibody [EPR7130(B)] - BSA and Azide free (ab207567)
    • Alexa Fluor® 488 Anti-TSG101 antibody [EPR7130(B)] (ab207663)
    • Alexa Fluor® 647 Anti-TSG101 antibody [EPR7130(B)] (ab207664)
    • PE Anti-TSG101 antibody [EPR7130(B)] (ab209927)
    • Alexa Fluor® 555 Anti-TSG101 antibody [EPR7130(B)] (ab214404)
    • Anti-TSG101 antibody [EPR7130(B)] - Low endotoxin, Azide free (ab216447)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat cytoplasmic extract lysate (ab14842)
    • Mouse brain tissue lysate - total protein (ab30151)
    • Mouse brain tissue lysate - total protein (0 days) (ab7188)
    • Mouse brain tissue lysate - total protein (14 days) (ab7189)
    • Mouse brain tissue lysate - total protein (7 days) (ab7190)
    • Jurkat whole cell lysate (ab7899)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab125011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
WB
Mouse
Human
Application Abreviews Notes
WB (5)
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).
Flow Cyt
1/40.

For unpurified use at 1/3.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF (3)
1/100.

For unpurified use at 1/10.

IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/30.

Notes
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).
Flow Cyt
1/40.

For unpurified use at 1/3.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
1/100.

For unpurified use at 1/10.

IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/30.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses.
    • Tissue specificity

      Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.
    • Sequence similarities

      Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.
      Contains 1 SB (steadiness box) domain.
      Contains 1 UEV (ubiquitin E2 variant) domain.
    • Domain

      The UEV domain is required for the interaction of the complex with ubiquitin. It also mediates the interaction with PTAP/PSAP motifs of HIV-1 P6 protein and human spumaretrovirus Gag protein.
      The coiled coil domain may interact with stathmin.
      The UEV domain binds ubiquitin and P-[ST]-A-P peptide motif independently.
    • Post-translational
      modifications

      Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.
    • Cellular localization

      Cytoplasm. Membrane. Nucleus. Late endosome membrane. Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Depending on the stage of the cell cycle, detected in the nucleus. Colocalized with CEP55 in the midbody during cytokinesis.
    • Target information above from: UniProt accession Q99816 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7251 Human
      • Entrez Gene: 22088 Mouse
      • Entrez Gene: 292925 Rat
      • Omim: 601387 Human
      • SwissProt: Q99816 Human
      • SwissProt: Q61187 Mouse
      • SwissProt: Q6IRE4 Rat
      • Unigene: 523512 Human
      • Unigene: 241334 Mouse
      • Unigene: 7410 Rat
      see all
    • Alternative names

      • ESCRT I complex subunit TSG101 antibody
      • ESCRT-I complex subunit TSG101 antibody
      • TS101_HUMAN antibody
      • TSG 10 antibody
      • TSG 101 antibody
      • TSG10 antibody
      • Tsg101 antibody
      • Tumor susceptibility 101 antibody
      • Tumor susceptibility gene 10 antibody
      • Tumor susceptibility gene 101 antibody
      • Tumor susceptibility gene 101 protein antibody
      • Tumor susceptibility protein antibody
      • Tumor susceptibility protein isoform 3 antibody
      • VPS 23 antibody
      • VPS23 antibody
      see all

    Images

    • Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
      Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
      All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) at 1/1000 dilution (unpurified)

      Lane 1 : Human brain lysate
      Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) lysate
      Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
      Lane 4 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 44 kDa
      Observed band size: 45 kDa why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
      Immunocytochemistry/ Immunofluorescence - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

      Immunocytochemsitry/Immunofluorescence analysis of HEK-293 (human epithelial cell line from embryonic kidney) cells labeling TSG101 (red) with unpurified ab125011 at 1/10. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

      Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling TSG101 with unpurified ab125011 at 1/30. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

    • Flow Cytometry - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
      Flow Cytometry - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

      Flow cytometric analysis of permeabilized K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling TSG101 (red) with unpurified ab125011 at 1/10. Green - Isotype control, rabbit monoclonal IgG.

    • OI-RD Scanning - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
      OI-RD Scanning - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
      Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
      All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) at 1/1000 dilution (unpurified)

      Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
      Lane 2 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 44 kDa
      Observed band size: 45 kDa why is the actual band size different from the predicted?



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
      Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
      All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) at 1/10000 dilution (purified)

      Lane 1 : Jurkat cell lysate
      Lane 2 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 44 kDa
      Observed band size: 45 kDa why is the actual band size different from the predicted?



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling TSG101 with purified ab125011 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

    • Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
      Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
      All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) at 1/500 dilution (unpurified)

      Lane 1 : Mouse heart tissue lysate
      Lane 2 : Rat heart tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 44 kDa
      Observed band size: 45 kDa why is the actual band size different from the predicted?



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
      Immunocytochemistry/ Immunofluorescence - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

      Immunocytochemsitry/Immunofluorescence analysis of HEK-293 (human epithelial cell line from embryonic kidney) cells labeling TSG101 (red) with purified ab125011 at 1/100. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

    • Flow Cytometry - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
      Flow Cytometry - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

      Flow cytometry analysis of permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling TSG101 with unpurified ab125011 at 1/3 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.

    • Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
      Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
      All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) (purified)

      All lanes : Mouse heart tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 44 kDa
      Observed band size: 45 kDa why is the actual band size different from the predicted?



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Flow Cytometry - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
      Flow Cytometry - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

      Flow cytometry analysis of permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling TSG101 with purified ab125011 at 1/40 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.

    • Anti-TSG101 antibody [EPR7130(B)] (ab125011)
      Anti-TSG101 antibody [EPR7130(B)] (ab125011)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (99)

    Publishing research using ab125011? Please let us know so that we can cite the reference in this datasheet.

    ab125011 has been referenced in 99 publications.

    • Lin F  et al. Bladder cancer cell-secreted exosomal miR-21 activates the PI3K/AKT pathway in macrophages to promote cancer progression. Int J Oncol 56:151-164 (2020). PubMed: 31814034
    • Liang Y  et al. Characterization of protein, long noncoding RNA and microRNA signatures in extracellular vesicles derived from resting and degranulated mast cells. J Extracell Vesicles 9:1697583 (2020). PubMed: 31853339
    • Han M  et al. Exosome-transmitted miR-567 reverses trastuzumab resistance by inhibiting ATG5 in breast cancer. Cell Death Dis 11:43 (2020). PubMed: 31969559
    • Brennan K  et al. A comparison of methods for the isolation and separation of extracellular vesicles from protein and lipid particles in human serum. Sci Rep 10:1039 (2020). PubMed: 31974468
    • Zhao Y  et al. Liver governs adipose remodelling via extracellular vesicles in response to lipid overload. Nat Commun 11:719 (2020). PubMed: 32024826
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-8 of 8 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TSG101 antibody [EPR7130(B)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (MC3T3 cells)
    Permeabilization
    Yes - NP40
    Specification
    MC3T3 cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 06 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TSG101 antibody [EPR7130(B)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U2OS)
    Permeabilization
    Yes - NP40
    Specification
    U2OS
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 06 2020

    Western blot abreview for Anti-TSG101 antibody [EPR7130(B)]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse vascular smooth muscle cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    25 µg
    Specification
    Mouse vascular smooth muscle cells
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 14 2020

    Western blot abreview for Anti-TSG101 antibody [EPR7130(B)]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U2OS)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    U2OS
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 14 2020

    Western blot abreview for Anti-TSG101 antibody [EPR7130(B)]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Bos taurus Purified protein (Extracellular vesicle (EV) enriched whey fraction)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris gel)
    Loading amount
    20 µg
    Specification
    Extracellular vesicle (EV) enriched whey fraction
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 27 2020

    Western blot abreview for Anti-TSG101 antibody [EPR7130(B)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U266)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    U266
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 02 2018

    Western blot abreview for Anti-TSG101 antibody [EPR7130(B)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - other (Exosomes from HK-2 cells)
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Loading amount
    50 µg
    Specification
    Exosomes from HK-2 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Mr. Eric Barros

    Verified customer

    Submitted Oct 24 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TSG101 antibody [EPR7130(B)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Breast Cancer Cell Line MDA-MB231)
    Permeabilization
    Yes - 0.1% Triton
    Specification
    Breast Cancer Cell Line MDA-MB231
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Mr. Gabriel Ortega

    Verified customer

    Submitted Mar 06 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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