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RecombinantRabMAb

Recombinant Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Reviews (10) Submit a question References (265)
Flow Cytometry - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
  • Immunocytochemistry/ Immunofluorescence - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
  • Immunocytochemistry/ Immunofluorescence - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
  • Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
  • Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
  • Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7130(B)] to TSG101
  • Suitable for: WB, Flow Cyt (Intra), ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 647 Carrier Free PE

Overview

  • Product name

    Anti-TSG101 antibody [EPR7130(B)]
    See all TSG101 primary antibodies

  • Description

    Rabbit monoclonal [EPR7130(B)] to TSG101

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt (Intra), ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human TSG101 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • WB: Mouse heart tissue lysates. K-562, Jurkat, SH-SY5Y, NIH/3T3 and PC-12 whole cell lysate. Mouse and rat heart and brain lysate. Flow Cyt: HeLa cells. ICC/IF: Jurkat cells and HeLa cells. IHC-P: Rat kidney tissue, mouse kidney tissue, human thyroid cancer tissue, human kidney tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab125011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (7)
1/1000 - 1/10000. Detects a band of approximately 47, 52 kDa (predicted molecular weight: 44 kDa).
Flow Cyt (Intra)
1/20.

For unpurified use at 1/3.  ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF (3)
Use a concentration of 1 µg/ml.

For unpurified use at 1/10.

This product gave a positive signal in HeLa fixed with 100% methanol (5 min).
IHC-P
1/300. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/30.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 47, 52 kDa (predicted molecular weight: 44 kDa).
Flow Cyt (Intra)
1/20.

For unpurified use at 1/3.  ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
Use a concentration of 1 µg/ml.

For unpurified use at 1/10.

This product gave a positive signal in HeLa fixed with 100% methanol (5 min).
IHC-P
1/300. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/30.

Target

  • Function

    Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses.

  • Tissue specificity

    Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.

  • Sequence similarities

    Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.
    Contains 1 SB (steadiness box) domain.
    Contains 1 UEV (ubiquitin E2 variant) domain.

  • Domain

    The UEV domain is required for the interaction of the complex with ubiquitin. It also mediates the interaction with PTAP/PSAP motifs of HIV-1 P6 protein and human spumaretrovirus Gag protein.
    The coiled coil domain may interact with stathmin.
    The UEV domain binds ubiquitin and P-[ST]-A-P peptide motif independently.

  • Post-translational
    modifications

    Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.

  • Cellular localization

    Cytoplasm. Membrane. Nucleus. Late endosome membrane. Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Depending on the stage of the cell cycle, detected in the nucleus. Colocalized with CEP55 in the midbody during cytokinesis.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ESCRT I complex subunit TSG101 antibody
    • ESCRT-I complex subunit TSG101 antibody
    • TS101_HUMAN antibody
    • TSG 10 antibody
    • TSG 101 antibody
    • TSG10 antibody
    • Tsg101 antibody
    • Tumor susceptibility 101 antibody
    • Tumor susceptibility gene 10 antibody
    • Tumor susceptibility gene 101 antibody
    • Tumor susceptibility gene 101 protein antibody
    • Tumor susceptibility protein antibody
    • Tumor susceptibility protein isoform 3 antibody
    • VPS 23 antibody
    • VPS23 antibody
    see all

Images

  • Flow Cytometry - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    Flow Cytometry - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling TSG101 with Purified ab125011 at 1:20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunocytochemistry/ Immunofluorescence - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    Immunocytochemistry/ Immunofluorescence - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

    ab125011 staining TSG101 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab125011 at 1 μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin at 0.5 μg/ml. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150119, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647), pre-adsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Immunocytochemistry/ Immunofluorescence - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    Immunocytochemistry/ Immunofluorescence - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    Immunocytochemistry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling TSG101 with Purified ab125011 at 1:50 dilution (4.5 µg/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling TSG101 with Purified ab125011 at 1:300 dilution (0.73 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling TSG101 with Purified ab125011 at 1:300 dilution (0.73 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid cancer tissue sections labeling TSG101 with Purified ab125011 at 1:300 dilution (0.73 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling TSG101 with Purified ab125011 at 1:300 dilution (0.73 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
  • Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) at 1/1000 dilution

    Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
    Lane 3 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 6 : Mouse heart lysate
    Lane 7 : Rat heart lysate
    Lane 8 : Mouse brain lysate
    Lane 9 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa
    Observed band size: 47, 52 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    The doublet are consistent with what have been described in the literature (PMID: 27882925, 31684046, 32932791, 11427703). 

    Image produced using the purified version.

  • Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

    Different batches of ab125011 were tested on Mouse heart lysate at 1.0 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 45 kDa.

    This data was generarted using the unpurified format.

  • Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    Anti-TSG101 antibody [EPR7130(B)] (ab125011)

References (265)

Publishing research using ab125011? Please let us know so that we can cite the reference in this datasheet.

ab125011 has been referenced in 265 publications.

  • Xie H  et al. Exosome-transmitted circVMP1 facilitates the progression and cisplatin resistance of non-small cell lung cancer by targeting miR-524-5p-METTL3/SOX2 axis. Drug Deliv 29:1257-1271 (2022). WB ; Human . PubMed: 35467477
  • Zhang J  et al. Potential Networks Regulated by MSCs in Acute-On-Chronic Liver Failure: Exosomal miRNAs and Intracellular Target Genes. Front Genet 12:650536 (2021). PubMed: 33968135
  • Li GQ  et al. MicroRNA-21 from bone marrow mesenchymal stem cell-derived extracellular vesicles targets TET1 to suppress KLF4 and alleviate rheumatoid arthritis. Ther Adv Chronic Dis 12:20406223211007369 (2021). PubMed: 33995992
  • Yu L  et al. Correlation between steroid levels in follicular fluid and hormone synthesis related substances in its exosomes and embryo quality in patients with polycystic ovary syndrome. Reprod Biol Endocrinol 19:74 (2021). PubMed: 34001150
  • Mao K  et al. Proteomics of extracellular vesicles in plasma reveals the characteristics and residual traces of COVID-19 patients without underlying diseases after 3 months of recovery. Cell Death Dis 12:541 (2021). PubMed: 34035220
View all Publications for this product

Customer reviews and Q&As

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1-10 of 10 Abreviews or Q&A

Western blot abreview for Anti-TSG101 antibody [EPR7130(B)]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (hMSC)
Gel Running Conditions
Reduced Denaturing (12.5)
Loading amount
10 µg
Specification
hMSC
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 20 2021

Western blot abreview for Anti-TSG101 antibody [EPR7130(B)]

 Average
Abreviews
abreview image
Application
Western blot
Sample
Bos taurus Cell lysate - other (exosomes)
Gel Running Conditions
Reduced Denaturing (4-12% Nupage Gel)
Loading amount
10 µg
Specification
exosomes
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Paula Mitchell

Verified customer

Submitted Jun 03 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-TSG101 antibody [EPR7130(B)]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MC3T3 cells)
Permeabilization
Yes - NP40
Specification
MC3T3 cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 06 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-TSG101 antibody [EPR7130(B)]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
Yes - NP40
Specification
U2OS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 06 2020

Western blot abreview for Anti-TSG101 antibody [EPR7130(B)]

 Average
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse vascular smooth muscle cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Mouse vascular smooth muscle cells
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 14 2020

Western blot abreview for Anti-TSG101 antibody [EPR7130(B)]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
U2OS
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 14 2020

Western blot abreview for Anti-TSG101 antibody [EPR7130(B)]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Bos taurus Purified protein (Extracellular vesicle (EV) enriched whey fraction)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris gel)
Loading amount
20 µg
Specification
Extracellular vesicle (EV) enriched whey fraction
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 27 2020

Western blot abreview for Anti-TSG101 antibody [EPR7130(B)]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (U266)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
U266
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 02 2018

Western blot abreview for Anti-TSG101 antibody [EPR7130(B)]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - other (Exosomes from HK-2 cells)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
50 µg
Specification
Exosomes from HK-2 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Eric Barros

Verified customer

Submitted Oct 24 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-TSG101 antibody [EPR7130(B)]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Breast Cancer Cell Line MDA-MB231)
Permeabilization
Yes - 0.1% Triton
Specification
Breast Cancer Cell Line MDA-MB231
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Gabriel Ortega

Verified customer

Submitted Mar 06 2017

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