Name: Anti-TSG101 antibody [EPR7130(B)]
Brand: RabMAb
SKU: ab125011
Rating: 5 (3 reviews)

Product name

Anti-TSG101 antibody [EPR7130(B)]
See all TSG101 primary antibodies
Description

Rabbit monoclonal [EPR7130(B)] to TSG101
Host species

Rabbit
Tested applications

Suitable for: WB, Flow Cyt, ICC/IF, IHC-Pmore details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human TSG101 aa 1-100. The exact sequence is proprietary.
Positive control
- WB: K562, Jurkat, and SH-SY5Y cell lysates. Human brain lysate; mouse and rat heart tissue lysates. IHC-P: Human kidney tissue. ICC/IF: HEK-293 cells. Flow Cyt: HeLa and K562 cells.
General notes

Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor^® 488 (ab150077).
See other anti-rabbit secondary antibodies that can be used with this antibody.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Dissociation constant (K_D)

K_D = 4.00 x 10 ^-12 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR7130(B)
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab125011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).
Flow Cyt		1/40. For unpurified use at 1/3. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF		1/100. For unpurified use at 1/10.
IHC-P		1/500. See IHC antigen retrieval protocols. For unpurified use at 1/30.

Application notes

Is unsuitable for IP.

Function

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses.
Tissue specificity

Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.
Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.
Contains 1 SB (steadiness box) domain.
Contains 1 UEV (ubiquitin E2 variant) domain.
Domain

The UEV domain is required for the interaction of the complex with ubiquitin. It also mediates the interaction with PTAP/PSAP motifs of HIV-1 P6 protein and human spumaretrovirus Gag protein.
The coiled coil domain may interact with stathmin.
The UEV domain binds ubiquitin and P-[ST]-A-P peptide motif independently.
Post-translational
modifications

Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.
Cellular localization

Cytoplasm. Membrane. Nucleus. Late endosome membrane. Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Depending on the stage of the cell cycle, detected in the nucleus. Colocalized with CEP55 in the midbody during cytokinesis.
Target information above from: UniProt accession Q99816 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 7251 Human
- Entrez Gene: 22088 Mouse
- Entrez Gene: 292925 Rat
- Omim: 601387 Human
- SwissProt: Q99816 Human
- SwissProt: Q61187 Mouse
- SwissProt: Q6IRE4 Rat
- Unigene: 523512 Human
- Unigene: 241334 Mouse
- Unigene: 7410 Rat
see all
Alternative names
- ESCRT I complex subunit TSG101 antibody
- ESCRT-I complex subunit TSG101 antibody
- TS101_HUMAN antibody
- TSG 10 antibody
- TSG 101 antibody
- TSG10 antibody
- Tsg101 antibody
- Tumor susceptibility 101 antibody
- Tumor susceptibility gene 10 antibody
- Tumor susceptibility gene 101 antibody
- Tumor susceptibility gene 101 protein antibody
- Tumor susceptibility protein antibody
- Tumor susceptibility protein isoform 3 antibody
- VPS 23 antibody
- VPS23 antibody
see all

Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) at 1/1000 dilution (unpurified)

Lane 1 : Human brain lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 44 kDa
Observed band size: 45 kDa
why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Immunocytochemsitry/Immunofluorescence analysis of HEK-293 (human epithelial cell line from embryonic kidney) cells labeling TSG101 (red) with unpurified ab125011 at 1/10. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling TSG101 with unpurified ab125011 at 1/30. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) at 1/1000 dilution (unpurified)

Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) at 1/10000 dilution (purified)

Lane 1 : Jurkat cell lysate
Lane 2 : SH-SY5Y cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Flow Cytometry - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Flow cytometric analysis of permeabilized K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling TSG101 (red) with unpurified ab125011 at 1/10. Green - Isotype control, rabbit monoclonal IgG.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling TSG101 with purified ab125011 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) at 1/500 dilution (unpurified)

Lane 1 : Mouse heart tissue lysate
Lane 2 : Rat heart tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Immunocytochemistry/ Immunofluorescence - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Immunocytochemsitry/Immunofluorescence analysis of HEK-293 (human epithelial cell line from embryonic kidney) cells labeling TSG101 (red) with purified ab125011 at 1/100. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).
Flow Cytometry - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Flow cytometry analysis of permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling TSG101 with unpurified ab125011 at 1/3 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.
Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) (purified)

All lanes : Mouse heart tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Flow Cytometry - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Flow cytometry analysis of permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling TSG101 with purified ab125011 at 1/40 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.
Other - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

This product has been referenced in:

Karttunen J et al. Precipitation-based extracellular vesicle isolation from rat plasma co-precipitate vesicle-free microRNAs. J Extracell Vesicles 8:1555410 (2019). Read more (PubMed: 30574280) »
Cui H et al. Macrophage-Derived miRNA-Containing Exosomes Induce Peritendinous Fibrosis after Tendon Injury through the miR-21-5p/Smad7 Pathway. Mol Ther Nucleic Acids 14:114-130 (2018). Read more (PubMed: 30594070) »

See all 23 Publications for this product

Publishing research using ab125011? Please let us know so that we can cite the reference in this datasheet.

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Application

Western blot

Sample

Human Cell lysate - whole cell (U266)

Gel Running Conditions

Reduced Denaturing

Loading amount

20 µg

Specification

U266

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Nov 02 2018

Application

Western blot

Sample

Human Cell lysate - other (Exosomes from HK-2 cells)

Gel Running Conditions

Reduced Denaturing (10% gel)

Loading amount

50 µg

Specification

Exosomes from HK-2 cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Mr. Eric Barros

Verified customer

Submitted Oct 24 2017

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (Breast Cancer Cell Line MDA-MB231)

Permeabilization

Yes - 0.1% Triton

Specification

Breast Cancer Cell Line MDA-MB231

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 22°C

Fixative

Paraformaldehyde

Mr. Gabriel Ortega

Verified customer

Submitted Mar 06 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Overview

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Datasheets and documents

References

This product has been referenced in:

Customer reviews and Q&As

Western blot abreview for Anti-TSG101 antibody [EPR7130(B)]

Western blot abreview for Anti-TSG101 antibody [EPR7130(B)]

Immunocytochemistry/ Immunofluorescence abreview for Anti-TSG101 antibody [EPR7130(B)]