Overview

  • Product name
    Anti-TTF1 antibody [EP1584Y]
    See all TTF1 primary antibodies
  • Description
    Rabbit monoclonal [EP1584Y] to TTF1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, IHC-Frmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TTF1 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P43699
    (Peptide available as ab187893)

  • Positive control
    • ICC/IF: Human thyroid cells; Rat bronchi cells. IHC-P: Human lung carcinoma and thyroid carcinoma tissue; Rat lung tissue; Mouse lung tissue. WB: HeLa and HEK-293 cell lysate; Rat and mouse lung lysate. Flow Cyt: HeLa and A549 cells.
  • General notes

      

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EP1584Y
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB 1/2000. Detects a band of approximately 38-42 kDa (predicted molecular weight: 38-42 kDa).Can be blocked with TTF1 peptide (ab187893).

For unpurified use at 1/500- 1/1000.

IHC-Fr 1/400.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis.
    • Tissue specificity
      Thyroid and lung.
    • Involvement in disease
      Defects in NKX2-1 are the cause of benign hereditary chorea (BHC) [MIM:118700]; also known as hereditary chorea without dementia. BHC is an autosomal dominant movement disorder. The early onset of symptoms (usully before the age of 5) and the observation that in some BHC families the symptoms tend to decrease in adulthood suggests that the disorder results from a developmental disturbance of the brain. BHC is non-progressive and patients have normal or slightly below normal intelligence. There is considerable inter- and intrafamilial variability, including dysarthria, axial distonia and gait disturbances.
      Defects in NKX2-1 are the cause of choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD) [MIM:610978]. This syndrome include neurological, thyroid, and respiratory problems.
    • Sequence similarities
      Belongs to the NK-2 homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Post-translational
      modifications
      Phosphorylated on serine residues.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • AV026640 antibody
      • BCH antibody
      • Benign chorea antibody
      • BHC antibody
      • Homeobox protein NK 2 homolog A antibody
      • Homeobox protein NK-2 homolog A antibody
      • Homeobox protein Nkx 2.1 antibody
      • Homeobox protein Nkx-2.1 antibody
      • Homeobox protein Nkx2.1 antibody
      • NK 2 antibody
      • NK 2 homolog A antibody
      • NK2 antibody
      • NK2 homeobox 1 antibody
      • NK2, drosophila, homolog of, A antibody
      • NK2.1, mouse, homolog of antibody
      • Nkx 2 1 antibody
      • NKX 2.1 antibody
      • NKX 2A antibody
      • NKX2 1 antibody
      • Nkx2-1 antibody
      • NKX2.1 antibody
      • NKX21_HUMAN antibody
      • NKX2A antibody
      • T EBP antibody
      • T/EBP antibody
      • TEBP antibody
      • Thyroid nuclear factor 1 antibody
      • Thyroid nuclear factor antibody
      • Thyroid specific enhancer binding protein antibody
      • Thyroid transcription factor 1 antibody
      • Tin man antibody
      • Tinman antibody
      • TITF 1 antibody
      • TITF1 antibody
      • TTF 1 antibody
      • TTF-1 antibody
      • TTF1 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence analysis of human thyroid cells labeling TTF1  with purified ab76013 at 1/500 dilution (0.3μg/ml). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.2% tritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). ab150077, a Goat anti rabbit IgG(Alexa Fluor® 488) secondary antibody was used at 1/1000 dilution. PBS instead of the primary antibody was used as a control. DAPI nuclear staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling TTF1 with purified ab76013 at 1/250 dilution (0.6 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.

    • Anti-TTF1 antibody [EP1584Y] (ab76013) at 1/10000 dilution (purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size: 38-42 kDa
      Observed band size: 40 kDa
      why is the actual band size different from the predicted?



      Blocking and diluting buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue sections labeling TTF1 with purified ab76013 at 1/250 dilution (0.6 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.

    • Anti-TTF1 antibody [EP1584Y] (ab76013) (purified) + Rat lung lysate at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 38-42 kDa
      Observed band size: 40 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue sections labeling TTF1 with purified ab76013 at 1/250 dilution (0.6 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.

    • Anti-TTF1 antibody [EP1584Y] (ab76013) at 1/2000 dilution (purified) + Mouse lung lysate at 20 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size: 38-42 kDa
      Observed band size: 40 kDa why is the actual band size different from the predicted?

    • Anti-TTF1 antibody [EP1584Y] (ab76013) at 1/2000 dilution (unpurified) + HEK293 whole cell lysate at 30 µg

      Secondary
      HRP-conjugated goat anti-rabbit polyclonal IgG at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 38-42 kDa
      Observed band size: 40 kDa why is the actual band size different from the predicted?


      Exposure time: 2 minutes


      Blocked with 5% milk

      See Abreview

    • Immunofluorescence analysis of rat bronchi cells, staining TTF1 with unpurified ab76013.

      Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 27°C. Samples were incubated with primary antibody (1/500 in blocking buffer) for 1 hour at 16°C. An AlexaFluor®594-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.

      See Abreview

    • Unpurified ab76013 staining TTF1 in murine fetal lung tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in formaldehyde and permeabilized using PBST. Samples were then blocked using 1.5% serum for 20 minutes at 25°C, then incubated with ab76013 at a 1/400 dilution for 12 hours at 4°C. A biotin conjugated goat anti-rabbit IgG was used as the secondary at a 1/200 dilution.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling TTF1 with purified ab76013 at 1/250 dilution (0.6 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.

    References

    This product has been referenced in:
    • Hou Z  et al. Wnt/Fgf crosstalk is required for the specification of basal cells in the mouse trachea. Development 146:N/A (2019). Read more (PubMed: 30696710) »
    • Wang GZ  et al. The Aryl hydrocarbon receptor mediates tobacco-induced PD-L1 expression and is associated with response to immunotherapy. Nat Commun 10:1125 (2019). Read more (PubMed: 30850589) »
    See all 55 Publications for this product

    Customer reviews and Q&As

    1-10 of 14 Abreviews or Q&A

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Ferret Tissue sections (Lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium citrate buffer (10 mM Sodium citrate, 0.05% Tween 20, pH 6.0)
    Permeabilization
    Yes - 0.5% Triton
    Specification
    Lung
    Blocking step
    2%BSA, 5%Normal Donkey Serum(NDS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
    Fixative
    Paraformaldehyde

    Mrs. Jinghui Gao

    Verified customer

    Submitted Oct 11 2018

    This product is known to not work in this application or species.
    Application
    Flow Cytometry
    Sample
    Human Cell (Differentiated hNSCs)
    Permeabilization
    Yes - 0.25% Triton X-100 in DPBS
    Gating Strategy
    Undifferentiated Stem Cells (white)
    Specification
    Differentiated hNSCs
    Preparation
    Cell harvesting/tissue preparation method: Accutase
    Sample buffer: PBS
    Fixation
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Sep 09 2015

    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
    Sample
    Human Cell (Differentiated hNSCs)
    Specification
    Differentiated hNSCs
    Permeabilization
    Yes - 0.25% Triton X-100 in DPBS
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Feb 13 2015

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (MLE12 cells)
    Loading amount
    20 µg
    Specification
    MLE12 cells
    Treatment
    TTF-1 Plasmid (2ug)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Feb 20 2013

    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mouse bronchiolar epithelium)
    Specification
    Mouse bronchiolar epithelium
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 27°C

    Abcam user community

    Verified customer

    Submitted Nov 23 2012

    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Rat Bronchi)
    Specification
    Rat Bronchi
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 27°C

    Abcam user community

    Verified customer

    Submitted Nov 07 2012

    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Frozen sections of human lung parenchyme)
    Specification
    Frozen sections of human lung parenchyme
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 27°C

    Abcam user community

    Verified customer

    Submitted Nov 07 2012

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Whole Lung)
    Loading amount
    30 µg
    Specification
    Whole Lung
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Nov 05 2012

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293)
    Loading amount
    30 µg
    Specification
    HEK293
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Nov 05 2012

    Answer

    For an anti-rabbit IgG secondary conjugated to APC (Allophycocyanin), we recommend ab130805:

    Click here (or use the following: https://www.abcam.com/index.html?datasheet=130805).

    Please let me know if you have any questions.

    Read More

    1-10 of 14 Abreviews or Q&A

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