Recombinant Anti-TTF1 antibody [SP141] (ab227652)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP141] to TTF1
- Suitable for: Flow Cyt, IHC-P, Flow Cyt (Intra), ICC/IF, IHC-Fr
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-TTF1 antibody [SP141]
See all TTF1 primary antibodies -
Description
Rabbit monoclonal [SP141] to TTF1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-P, Flow Cyt (Intra), ICC/IF, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human thyroid, lung, lung carcinoma, Mouse lung, and Rat lung tissue; Flow Cyt: HeLa cells. Flow Cyt (Intra): Human thyroid.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP141 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227652 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
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IHC-P |
1/100.
Perform heat mediated antigen retrieval with 1 mM EDTA buffer pH 8.0 before commencing with IHC staining protocol. Primary antibody incubation for 30 minutes at room temperature. |
|
Flow Cyt (Intra) |
1/100.
Primary antibody incubation for 30 minutes at 4°C. |
|
ICC/IF |
1/25.
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IHC-Fr |
1/20.
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Notes |
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Flow Cyt
Use at an assay dependent concentration. |
IHC-P
1/100. Perform heat mediated antigen retrieval with 1 mM EDTA buffer pH 8.0 before commencing with IHC staining protocol. Primary antibody incubation for 30 minutes at room temperature. |
Flow Cyt (Intra)
1/100. Primary antibody incubation for 30 minutes at 4°C. |
ICC/IF
1/25. |
IHC-Fr
1/20. |
Target
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Function
Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. -
Tissue specificity
Thyroid and lung. -
Involvement in disease
Defects in NKX2-1 are the cause of benign hereditary chorea (BHC) [MIM:118700]; also known as hereditary chorea without dementia. BHC is an autosomal dominant movement disorder. The early onset of symptoms (usully before the age of 5) and the observation that in some BHC families the symptoms tend to decrease in adulthood suggests that the disorder results from a developmental disturbance of the brain. BHC is non-progressive and patients have normal or slightly below normal intelligence. There is considerable inter- and intrafamilial variability, including dysarthria, axial distonia and gait disturbances.
Defects in NKX2-1 are the cause of choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD) [MIM:610978]. This syndrome include neurological, thyroid, and respiratory problems. -
Sequence similarities
Belongs to the NK-2 homeobox family.
Contains 1 homeobox DNA-binding domain. -
Post-translational
modificationsPhosphorylated on serine residues. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7080 Human
- Entrez Gene: 21869 Mouse
- Entrez Gene: 25628 Rat
- Omim: 600635 Human
- SwissProt: P43699 Human
- SwissProt: P50220 Mouse
- SwissProt: P23441 Rat
- Unigene: 94367 Human
see all -
Alternative names
- AV026640 antibody
- BCH antibody
- Benign chorea antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat lung tissue sections labeling TTF1 with ab227652 at 1/100 dilution (1.70 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse lung tissue sections labeling TTF1 with ab227652 at 1/100 dilution (1.70 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid tissue sections labeling TTF1 with ab227652 at 1/100 dilution (1.70 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung tissue sections labeling TTF1 with ab227652 at 1/100 dilution (1.70 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Flow Cytometry analysis of TT (Human thyroid carcinoma epithelial cell) cells labeling TTF1 with purified ab227652 at 1:170 dilution (1 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.
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Immunocytochemistry/ Immunofluorescence analysis of TT (Human thyroid carcinoma epithelial cell) cells labeling TTF1 with purified ab227652 at 1:25 (6.8 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Immunohistochemistry (Frozen sections) analysis of rat lung tissue sections labeling TTF1 with purified ab227652 at 1:20 (8.5 µg/ml). Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Fixed with 4% paraformaldehyde and permeabilised using 0.2% Triton X-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control:PBS instead of the primary antibody. DAPI was used as a Nuclear stain.
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Formalin-fixed, paraffin-embedded human thyroid tissue stained for TTF1 using ab227652 at 1/100 dilution in immunohistochemical analysis.
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Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling TTF1 with ab227652 at 1/100 dilution (green) compared with a rabbit IgG negative control (blue).
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Formalin-fixed, paraffin-embedded human lung tissue stained for TTF1 using ab227652 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for TTF1 using ab227652 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab227652 has been referenced in 1 publication.
- Nunnelly LF et al. St18 specifies globus pallidus projection neuron identity in MGE lineage. Nat Commun 13:7735 (2022). PubMed: 36517477