Overview

  • Product name
    Anti-TTF1 antibody [SP141]
    See all TTF1 primary antibodies
  • Description
    Rabbit monoclonal [SP141] to TTF1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TTF1 aa 300 to the C-terminus (internal sequence). The exact sequence is proprietary.
    Database link: P43699

  • Positive control
    • IHC-P: Human thyroid, lung, lung carcinoma, Mouse lung, and Rat lung tissue; FC: HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.6
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity
    Protein A/G purified
  • Purification notes
    Purified from TCS by protein A/G.
  • Clonality
    Monoclonal
  • Clone number
    SP141
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100.

Primary antibody incubation for 30 minutes at 4°C.

IHC-P 1/100.

Perform heat mediated antigen retrieval with 1 mM EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Primary antibody incubation for 30 minutes at room temperature.

Target

  • Function
    Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis.
  • Tissue specificity
    Thyroid and lung.
  • Involvement in disease
    Defects in NKX2-1 are the cause of benign hereditary chorea (BHC) [MIM:118700]; also known as hereditary chorea without dementia. BHC is an autosomal dominant movement disorder. The early onset of symptoms (usully before the age of 5) and the observation that in some BHC families the symptoms tend to decrease in adulthood suggests that the disorder results from a developmental disturbance of the brain. BHC is non-progressive and patients have normal or slightly below normal intelligence. There is considerable inter- and intrafamilial variability, including dysarthria, axial distonia and gait disturbances.
    Defects in NKX2-1 are the cause of choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD) [MIM:610978]. This syndrome include neurological, thyroid, and respiratory problems.
  • Sequence similarities
    Belongs to the NK-2 homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Post-translational
    modifications
    Phosphorylated on serine residues.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AV026640 antibody
    • BCH antibody
    • Benign chorea antibody
    • BHC antibody
    • Homeobox protein NK 2 homolog A antibody
    • Homeobox protein NK-2 homolog A antibody
    • Homeobox protein Nkx 2.1 antibody
    • Homeobox protein Nkx-2.1 antibody
    • Homeobox protein Nkx2.1 antibody
    • NK 2 antibody
    • NK 2 homolog A antibody
    • NK2 antibody
    • NK2 homeobox 1 antibody
    • NK2, drosophila, homolog of, A antibody
    • NK2.1, mouse, homolog of antibody
    • Nkx 2 1 antibody
    • NKX 2.1 antibody
    • NKX 2A antibody
    • NKX2 1 antibody
    • Nkx2-1 antibody
    • NKX2.1 antibody
    • NKX21_HUMAN antibody
    • NKX2A antibody
    • T EBP antibody
    • T/EBP antibody
    • TEBP antibody
    • Thyroid nuclear factor 1 antibody
    • Thyroid nuclear factor antibody
    • Thyroid specific enhancer binding protein antibody
    • Thyroid transcription factor 1 antibody
    • Tin man antibody
    • Tinman antibody
    • TITF 1 antibody
    • TITF1 antibody
    • TTF 1 antibody
    • TTF-1 antibody
    • TTF1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat lung tissue sections labeling TTF1 with ab227652 at 1/100 dilution (1.70 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse lung tissue sections labeling TTF1 with ab227652 at 1/100 dilution (1.70 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid tissue sections labeling TTF1 with ab227652 at 1/100 dilution (1.70 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung tissue sections labeling TTF1 with ab227652 at 1/100 dilution (1.70 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Flow Cytometry analysis of TT (Human thyroid carcinoma epithelial cell) cells labeling TTF1 with purified ab227652 at 1:170 dilution (1 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.
  • Immunocytochemistry/ Immunofluorescence analysis of TT (Human thyroid carcinoma epithelial cell) cells labeling TTF1 with purified ab227652 at 1:25 (6.8 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Frozen sections) analysis of rat lung tissue sections labeling TTF1 with purified ab227652 at 1:20 (8.5 µg/ml). Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Fixed with 4% paraformaldehyde and permeabilised using 0.2% Triton X-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control:PBS instead of the primary antibody. DAPI was used as a Nuclear stain.
  • Formalin-fixed, paraffin-embedded human thyroid tissue stained for TTF1 using ab227652 at 1/100 dilution in immunohistochemical analysis.

  • Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling TTF1 with ab227652 at 1/100 dilution (green) compared with a rabbit IgG negative control (blue). 

  • Formalin-fixed, paraffin-embedded human lung tissue stained for TTF1 using ab227652 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for TTF1 using ab227652 at 1/100 dilution in immunohistochemical analysis.

References

ab227652 has not yet been referenced specifically in any publications.

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