Recombinant Anti-TUFM antibody [EPR12796(B)] (ab175199)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12796(B)] to TUFM
- Suitable for: Flow Cyt (Intra), IP, ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TUFM antibody [EPR12796(B)]
See all TUFM primary antibodies -
Description
Rabbit monoclonal [EPR12796(B)] to TUFM -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IP, ICC/IF, WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human TUFM aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P49411 -
Positive control
- HepG2, A431, HeLa and Jurkat whole cell lysate (ab7899)
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12796(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/2500.
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|
IP |
1/10 - 1/100.
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ICC/IF |
1/500.
For unpurified use at 1/50 - 1/100 |
|
WB | (1) |
1/10000 - 1/50000. Predicted molecular weight: 50 kDa.
|
Notes |
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Flow Cyt (Intra)
1/2500. |
IP
1/10 - 1/100. |
ICC/IF
1/500. For unpurified use at 1/50 - 1/100 |
WB
1/10000 - 1/50000. Predicted molecular weight: 50 kDa. |
Target
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Function
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. -
Involvement in disease
Combined oxidative phosphorylation deficiency 4 (COXPD4) [MIM:610678]: A mitochondrial disease resulting in neonatal lactic acidosis, rapidly progressive encephalopathy, severely decreased mitochondrial protein synthesis, and combined deficiency of mtDNA-related mitochondrial respiratory chain complexes. Note=The disease is caused by mutations affecting the gene represented in this entry. -
Sequence similarities
Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 7284 Human
- Entrez Gene: 233870 Mouse
- Entrez Gene: 293481 Rat
- Omim: 602389 Human
- SwissProt: P49411 Human
- SwissProt: Q8BFR5 Mouse
- SwissProt: P85834 Rat
- Unigene: 12084 Human
see all -
Alternative names
- COXPD4 antibody
- EF Tu antibody
- EF TuMT antibody
see all
Images
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All lanes : Anti-TUFM antibody [EPR12796(B)] (ab175199) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 50 kDa -
Immunocytochemistry/Immunofluorescence analysis of HepG2 (human hepatocellular carcinoma) cells labelling TUFM with purified ab175199 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue).
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
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Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling TUFM (red) with purified ab175199 at a 1/2500 dilution (1ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
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Immunofluorescence analysis of HepG2 cells labeling TUFM with ab175199 at a 1/50 dilution (green). DAPI nuclear staining (blue).
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Western blot analysis on immunoprecipitation pellet from (Lane 1) HepG2 cells lysate or (Lane 2) 1X PBS (negative control) using ab175199 at a 1/10 dilution and HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab175199 has been referenced in 2 publications.
- Lee YG et al. LONP1 and ClpP cooperatively regulate mitochondrial proteostasis for cancer cell survival. Oncogenesis 10:18 (2021). PubMed: 33637676
- Viale A et al. Oncogene ablation-resistant pancreatic cancer cells depend on mitochondrial function. Nature : (2014). PubMed: 25119024