Recombinant Anti-Thiophosphate ester antibody [51-8] (ab133473)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [51-8] to Thiophosphate ester
- Suitable for: IP
- Reacts with: Species independent
Related conjugates and formulations
Overview
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Product name
Anti-Thiophosphate ester antibody [51-8]
See all Thiophosphate ester primary antibodies -
Description
Rabbit monoclonal [51-8] to Thiophosphate ester -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Thiophosphate ester conjugated to keyhole limpet haemocyanin.
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Positive control
- HeLa cell lysate
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General notes
This antibody was developed as part of a collaboration between Abcam and the lab of Dr. Kevan Shokat at University of California at San Francisco.
This antibody is useful for studying kinase-substrate relationships of interest. The antibody is also context independent in that it can recognize serine, threonine, and tyrosine thiophosphorylated residues in a multitude of kinase phosphorylation consensus motifs.
In order for this antibody to work in WB and IP, the customer needs to perform a specific kinase reaction using ATP gamma S and PNBM.
See specific Protocol at the bottom of this page for details.This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Primary antibody notes
This antibody is useful for studying kinase-substrate relationships of interest. The antibody is also context independent in that it can recognize serine, threonine, and tyrosine thiophosphorylated residues in a multitude of kinase phosphorylation consensus motifs.
In order for this antibody to work in WB and IP, the customer needs to perform a specific kinase reaction using ATP gamma S and PNBM.
See specific Protocol at the bottom of this page for details. -
Clonality
Monoclonal -
Clone number
51-8 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP | (1) |
1/5000.
Recommended: 0.5-1 ug of antibody per 1ug of kinase substrate |
Notes |
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IP
1/5000. Recommended: 0.5-1 ug of antibody per 1ug of kinase substrate |
Target
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Relevance
Thiophosphate ester is used as fungicide.
Images
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ab133473, at 1:5000, immunoblotting immunoprecipitated samples pre-resolved by SDS-PAGE. Myelin basic protein (MBP) was thiophosphorylated by AS GSK3 beta (GSK3 beta also catalyzes an auto thiophosporylation reaction). Samples were treated with or without p-nitrobenzyl mesylate (PNBM) for 1 hour at room temperature. PNBM was removed by size exclusion and 1mg/ml of HeLa cell lysate was added to samples.
ab133473 was pre-incubated with protein G beads and was added to sample for an immunoprecipitation at 4°C overnight:
(1) Thiophosphorylated MBP
(2) PNBM treated thiophosphorylated MBP sample
(3) HeLa cell lysate
(4) HeLa cell lysate with PNBM treated thiophosphorylated MBP sample
(5) Control
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab133473 has been referenced in 15 publications.
- Zhang H et al. A subcellular map of the human kinome. Elife 10:N/A (2021). PubMed: 33988507
- Schütz M et al. Functional Relevance of the Interaction between Human Cyclins and the Cytomegalovirus-Encoded CDK-Like Protein Kinase pUL97. Viruses 13:N/A (2021). PubMed: 34198986
- Dang F et al. Inhibition of CK1ε potentiates the therapeutic efficacy of CDK4/6 inhibitor in breast cancer. Nat Commun 12:5386 (2021). PubMed: 34508104
- Sullivan S et al. Regulation of plant phototropic growth by NPH3/RPT2-like substrate phosphorylation and 14-3-3 binding. Nat Commun 12:6129 (2021). PubMed: 34675214
- Hirschhäuser A et al. CK1α protects WAVE from degradation to regulate cell shape and motility in the immune response. J Cell Sci 134:N/A (2021). PubMed: 34730182