Overview

  • Product name

    Anti-Thorase antibody [N125/10]
    See all Thorase primary antibodies
  • Description

    Mouse monoclonal [N125/10] to Thorase
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein corresponding to Mouse Thorase aa 1-361.

  • Positive control

    • Rat brain lysate
  • General notes

    The clone number has been updated from S125-10 to N125/10, both clone numbers name the same antibody clone.

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    N125/10
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab94583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 40 kDa.
IHC-P 1/1000.
IHC-Fr Use a concentration of 0.1 - 1 µg/ml.
ICC/IF 1/100.

Target

  • Function

    ATPase that plays a critical role in regulating the surface expression of AMPA receptors (AMPAR), thereby regulating synaptic plasticity and learning and memory. Required for NMDA-stimulated AMPAR internalization and inhibition of GRIA1 and GRIA2 recycling back to the plasma membrane; these activities are ATPase-dependent.
  • Sequence similarities

    Belongs to the AAA ATPase family.
  • Cellular localization

    Peroxisome. Cell junction, synapse, postsynaptic cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4921525H23Rik antibody
    • AAA+ ATPase Thorase antibody
    • Ab2-088 antibody
    • AFDC1 antibody
    • Atad1 antibody
    • ATAD1_HUMAN antibody
    • ATPase family AAA domain containing protein 1 antibody
    • ATPase family AAA domain-containing protein 1 antibody
    • ATPase family, AAA domain containing 1 antibody
    • ATPase Thorase antibody
    • AW107648 antibody
    • FLJ14600 antibody
    • FNP001 antibody
    • Thorase antibody
    see all

Images

  • Western blot detection of Thorase in Rat brain membrane tissues using ab94583 at 1:1000 dilution.

  • Ice cold methanol-fixed HaCaT cells stained for Thorase (green) using ab94583 at 1/100 dilution in ICC/IF. FITC Goat Anti-Mouse (green) was used as the secondary at 1/50 dilution. Cytoplasmic and accumulates around nucleus is observed.

  • Bouin's-fixed, paraffin-embedded mouse backskin tissue stained for Thorase (green) using ab94583 at 1/1oo dilution in immunohistochemical analysis. Goat Anti-Mouse (green) was used as the secondary antibody at 1/50 dilution. Nuclear staining in all basal cells is obsereved.

References

ab94583 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Loading amount
25 µg
Specification
293T
Treatment
siRNA knockdown
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 08 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up