Anti-Thrombomodulin antibody (ab230189)
Key features and details
- Rabbit polyclonal to Thrombomodulin
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Thrombomodulin antibody
See all Thrombomodulin primary antibodies -
Description
Rabbit polyclonal to Thrombomodulin -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human Thrombomodulin aa 200-500.
Database link: P07204 -
Positive control
- WB: Rat heart tissue lysate; mouse heart, kidney and brain tissue lysates; HepG2, A549 and A431 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
- Hep G2 whole cell lysate (ab166833)
- Mouse kidney normal tissue lysate - total protein (ab29305)
- A-431 whole cell lysate (ab30132)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse heart normal tissue lysate - total protein (ab30291)
- Mouse brain tissue lysate - total protein (ab4022)
- A-431 whole cell lysate (ab7909)
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). |
Target
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Function
Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated. -
Tissue specificity
Endothelial cells are unique in synthesizing thrombomodulin. -
Involvement in disease
Defects in THBD are the cause of thrombophilia due to thrombomodulin defect (THR-THBD) [MIM:188040]. A hemostatic disorder characterized by a tendency to thrombosis.
Defects in THBD are a cause of susceptibility to hemolytic uremic syndrome atypical type 6 (AHUS6) [MIM:612926]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. -
Sequence similarities
Contains 1 C-type lectin domain.
Contains 6 EGF-like domains. -
Post-translational
modificationsN-glycosylated.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 7056 Human
- Entrez Gene: 21824 Mouse
- Entrez Gene: 83580 Rat
- Omim: 188040 Human
- SwissProt: P07204 Human
- SwissProt: P15306 Mouse
- Unigene: 2030 Human
- Unigene: 24096 Mouse
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Alternative names
- AHUS 6 antibody
- AHUS6 antibody
- BDCA 3 antibody
see all
Images
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All lanes : Anti-Thrombomodulin antibody (ab230189) at 1/1000 dilution
Lane 1 : Rat heart lysate
Lane 2 : Mouse kidney lysate
Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 4 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Secondary
All lanes : Goat anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa -
All lanes : Anti-Thrombomodulin antibody (ab230189) at 1/1000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse brain lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Secondary
All lanes : Goat anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab230189 has been referenced in 1 publication.
- Shan X et al. Edoxaban improves atrial fibrillation and thromboembolism through regulation of the Wnt-ß-induced PI3K/ATK-activated protein C system. Exp Ther Med 17:3509-3517 (2019). PubMed: 30988731