Anti-Thrombospondin 2 antibody (ab112543)
Key features and details
- Rabbit polyclonal to Thrombospondin 2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Thrombospondin 2 antibody
See all Thrombospondin 2 primary antibodies -
Description
Rabbit polyclonal to Thrombospondin 2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to an internal sequence of Human Thrombospondin 2.
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Positive control
- WB: Hela, 22RV1, U-87MG, THP-1, PC-3, Caco-2 whole cell lysates. IHC-P: Human Placenta tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab112543 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 130 kDa.
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml.
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Notes |
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WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 130 kDa. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. |
Target
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Function
Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Can bind to fibrinogen, fibronectin, laminin and type V collagen. -
Tissue specificity
High expression in invertebral disk tissue. -
Involvement in disease
Genetic variations in THBS2 may be a cause of susceptibility to intervertebral disc disease (IDD) [MIM:603932]; also known as lumbar disk herniation (LDH). IDD is one of the most common musculo-skeletal disorders and the predominant cause of low-back pain and unilateral leg pain. -
Sequence similarities
Belongs to the thrombospondin family.
Contains 3 EGF-like domains.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 1 TSP N-terminal (TSPN) domain.
Contains 3 TSP type-1 domains.
Contains 8 TSP type-3 repeats.
Contains 1 VWFC domain. - Information by UniProt
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Database links
- Entrez Gene: 7058 Human
- Omim: 188061 Human
- SwissProt: P35442 Human
- Unigene: 371147 Human
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Alternative names
- THBS2 antibody
- Thrombospondin-2 antibody
- TSP2 antibody
- TSP2_HUMAN antibody
Images
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All lanes : Anti-Thrombospondin 2 antibody (ab112543) at 0.5 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : 22RV1 cell lysate
Lane 3 : U-87MG cell lysate
Lane 4 : THP-1 cell lysate
Lane 5 : PC-3 cell lysate
Lane 6 : Caco-2 cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG-HRP secondary antibody at 1/10000 dilution
Predicted band size: 130 kDa -
Thrombospondin 2/THBS2 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab112543 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab112543 has been referenced in 7 publications.
- Mo T et al. MicroRNA 1228 Mediates the Viability of High Glucose-Cultured Renal Tubule Cells through Targeting Thrombospondin 2 and PI3K/AKT Signaling Pathway. Kidney Blood Press Res 47:1-12 (2022). PubMed: 34784607
- Sun L et al. PD-L1 promotes myofibroblastic activation of hepatic stellate cells by distinct mechanisms selective for TGF-β receptor I versus II. Cell Rep 38:110349 (2022). PubMed: 35139382
- Zhang S et al. THBS2 is Closely Related to the Poor Prognosis and Immune Cell Infiltration of Gastric Cancer. Front Genet 13:803460 (2022). PubMed: 35186032
- Kim NH et al. A Pilot Study of Peritumor Administration of 5-FU for Preventing Bleeding in Advanced Gastric Cancer. Korean J Gastroenterol 80:273-280 (2022). PubMed: 36567441
- Zhang S et al. Bioinformatics Analysis of Hub Genes and Potential Therapeutic Agents Associated with Gastric Cancer. Cancer Manag Res 13:8929-8951 (2021). PubMed: 34876855
- Byrling J et al. Mass spectrometry-based analysis of formalin-fixed, paraffin-embedded distal cholangiocarcinoma identifies stromal thrombospondin-2 as a potential prognostic marker. J Transl Med 18:343 (2020). PubMed: 32887625
- Jellvert Å et al. Castration is a prerequisite for the inhibitory effect of metronomic chemotherapy on the growth of experimental castration-resistant prostate cancer. Acta Oncol 57:895-901 (2018). PubMed: 29299975