Anti-Thromboxane synthase antibody (ab39362)
Key features and details
- Rabbit polyclonal to Thromboxane synthase
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Thromboxane synthase antibody
See all Thromboxane synthase primary antibodies -
Description
Rabbit polyclonal to Thromboxane synthase -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cynomolgus monkey -
Immunogen
Synthetic peptide:
TNPDCQEKLLREVDVFKEK
, corresponding to amino acids 359/377 of Human Thromboxane synthase -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab39362 is an affinity purified IgG -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab39362 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500. Detects a band of approximately 54 kDa (predicted molecular weight: 61 kDa).
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Notes |
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WB
1/500. Detects a band of approximately 54 kDa (predicted molecular weight: 61 kDa). |
Target
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Tissue specificity
Platelets, lung, kidney, spleen, macrophages and lung fibroblasts. -
Involvement in disease
Defects in TBXAS1 are the cause of Ghosal hematodiaphyseal dysplasia (GHDD) [MIM:231095]. GHDD is a rare autosomal recessive disorder characterized by increased bone density with predominant diaphyseal involvement and aregenerative corticosteroid-sensitive anemia. Aregenerative anemia is characterized by bone marrow failure, so that functional marrow cells are regenerated slowly or not at all.
Defects in TBXAS1 are the cause of thromboxane synthetase deficiency (TBXAS1 deficiency) [MIM:274180]. It is characterized by hemorrhagic diathesis. -
Sequence similarities
Belongs to the cytochrome P450 family. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 6916 Human
- Omim: 274180 Human
- SwissProt: Q2PG45 Cynomolgus monkey
- SwissProt: P24557 Human
- Unigene: 520757 Human
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Alternative names
- BDPLT14 antibody
- CYP5 antibody
- CYP5A1 antibody
see all
Images
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All lanes : Anti-Thromboxane synthase antibody (ab39362) at 1 µg/ml
Lane 1 : Human spleen tissue lysate - total protein (ab29699)
Lane 2 : WI38 (Human lung fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Additional bands at: 21 kDa, 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab39362 has been referenced in 9 publications.
- Pai CH et al. Lack of Thromboxane Synthase Prevents Hypertension and Fetal Growth Restriction after High Salt Treatment during Pregnancy. PLoS One 11:e0151617 (2016). IHC . PubMed: 26974824
- Walker MA et al. Vasoactive enzymes and blood flow responses to passive and active exercise in peripheral arterial disease. Atherosclerosis 246:98-105 (2016). PubMed: 26771386
- Font-Nieves M et al. Induction of COX-2 Enzyme and Down-regulation of COX-1 Expression by Lipopolysaccharide (LPS) Control Prostaglandin E2 Production in Astrocytes. J Biol Chem 287:6454-68 (2012). WB ; Mouse . PubMed: 22219191
- Chu LM et al. Effects of cyclooxygenase inhibition on cardiovascular function in a hypercholesterolemic swine model of chronic ischemia. Am J Physiol Heart Circ Physiol 302:H479-88 (2012). WB ; Pig . PubMed: 22037194
- Feng J et al. Thromboxane-induced contractile response of human coronary arterioles is diminished after cardioplegic arrest. Ann Thorac Surg 92:829-36 (2011). WB ; Human . PubMed: 21871266
- Carter PR et al. Thromboxane-prostanoid receptor expression and antagonism in dextran-sodium sulfate-induced colitis. Inflamm Res 60:87-92 (2011). IHC-Fr ; Mouse . PubMed: 20694740
- Shoeb M et al. Inhibition of aldose reductase prevents endotoxin-induced inflammation by regulating the arachidonic acid pathway in murine macrophages. Free Radic Biol Med 51:1686-96 (2011). WB ; Mouse . PubMed: 21856412
- Hansen AH et al. Exercise training alters the balance between vasoactive compounds in skeletal muscle of individuals with essential hypertension. Hypertension 58:943-9 (2011). PubMed: 21896936
- Wright WS et al. Expression of thromboxane synthase and the thromboxane-prostanoid receptor in the mouse and rat retina. Exp Eye Res 89:532-7 (2009). IHC-P ; Mouse, Rat . PubMed: 19523949