PerCP/Cy5.5® Anti-Thy1.2 antibody [30-H12] (ab218775)
Key features and details
- PerCP/Cy5.5® Rat monoclonal [30-H12] to Thy1.2
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
- Isotype: IgG2b
Overview
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Product name
PerCP/Cy5.5® Anti-Thy1.2 antibody [30-H12]
See all Thy1.2 primary antibodies -
Description
PerCP/Cy5.5® Rat monoclonal [30-H12] to Thy1.2 -
Host species
Rat -
Conjugation
PerCP/Cy5.5®. Ex: 482nm, Em: 690nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Flow Cyt: C57Bl/6 splenocytes.
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General notes
Thy1.2 is expressed on thymocytes, mature T cells and neurons in mouse strains that express the CD90.2 allele (BALB/c, CBA, C3H, C57BL/6, SJL and others). ab218775 does not react with the CD90.1 allele expressed in mouse strains such as PL and AKR.
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
ab218775 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. -
Clonality
Monoclonal -
Clone number
30-H12 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
Use 0.25µg per reaction. |
Notes |
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Flow Cyt
Use at an assay dependent concentration. Use 0.25µg per reaction. |
Target
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Function
May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 21838 Mouse
- SwissProt: P01831 Mouse
- Unigene: 3951 Mouse
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Alternative names
- CD90 antibody
- CD90 antigen antibody
- CD90.2 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab218775 has been referenced in 1 publication.
- Paris JR et al. BrdU Positive Cells Induced in a Genetic Mouse Model of Glaucoma. J Ophthalmol Vis Sci 6:N/A (2021). PubMed: 33829147