Anti-Thyroid Hormone Receptor beta antibody (ab5622)
Key features and details
- Rabbit polyclonal to Thyroid Hormone Receptor beta
- Suitable for: ICC/IF, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Thyroid Hormone Receptor beta antibody
See all Thyroid Hormone Receptor beta primary antibodies -
Description
Rabbit polyclonal to Thyroid Hormone Receptor beta -
Host species
Rabbit -
Specificity
This antibody does not detect TR alpha-1 or TRv alpha-2. -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Thyroid Hormone Receptor beta aa 62-81. With an N-terminal added cysteine.
Sequence:IFHLDHDDVNDQSVSSAQTF
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Positive control
- IHC-P: Human colon, Human thyroid, Rat thyroid tissues. ICC: HeLa, A431 and L6 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/50 - 1/500.
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IHC-P |
1/100 - 1/500.
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Notes |
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ICC/IF
1/50 - 1/500. |
IHC-P
1/100 - 1/500. |
Target
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Function
High affinity receptor for triiodothyronine. -
Involvement in disease
Defects in THRB are the cause of generalized thyroid hormone resistance (GTHR) [MIM:188570, 274300]. GTHR is transmitted as an autosomal dominant trait, but an autosomal recessive form also exists. The disease is characterized by goiter, abnormal mental functions, increased susceptibility to infections, abnormal growth and bone maturation, tachycardia and deafness. Affected individuals may also have attention deficit-hyperactivity disorders (ADHD) and language difficulties. GTHR patients also have high levels of circulating thyroid hormones (T3-T4), with normal or slightly elevated thyroid stimulating hormone (TSH).
Defects in THRB are the cause of selective pituitary thyroid hormone resistance (PRTH) [MIM:145650]; also known as familial hyperthyroidism due to inappropriate thyrotropin secretion. PRTH is a variant form of thyroid hormone resistance and is characterized by clinical hyperthyroidism, with elevated free thyroid hormones, but inappropriately normal serum TSH. Unlike GRTH, where the syndrome usually segregates with a dominant allele, the mode of inheritance in PRTH has not been established. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7068 Human
- Entrez Gene: 24831 Rat
- Omim: 190160 Human
- SwissProt: P10828 Human
- SwissProt: P18113 Rat
- Unigene: 187861 Human
- Unigene: 728126 Human
- Unigene: 88692 Rat
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Alternative names
- Avian erythroblastic leukemia viral (v erb a) oncogene homolog 2 antibody
- C ERBA 2 antibody
- C ERBA BETA antibody
see all
Images
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ab5622 labelling Thyroid Hormaone Receptor beta in the nucleus of Rat thyroid tissue (right) compared with a negative control (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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ab5622 labelling Thyroid Hormaone Receptor beta in the nucleus of Human thyroid tissue (right) compared with a negative control (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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ab5622 staining Thyroid Hormone Receptor in Human colon tissue sections (right) compared to negative control (left) by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 3% H2O2-methanol for 15 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/20) for 1 hour at 37°C. A HRP-conjugated secondary antibody was used for detection.
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ab5622 staining Thyroid Hormone Receptor beta in A431 cells (right) compared to negative control (left) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with forrmalin, permeabilized with 0.1% Triton X-100 in TBS and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100) overnight at 4°C. A Dylight-conjugated secondary antibody was used. F-actin stained with red phallodin (red) and nuclei stained with Hoechst (blue).
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ab5622 staining Thyroid Hormone Receptor beta in Hela cells (right) compared to negative control (left) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with forrmalin, permeabilized with 0.1% Triton X-100 in TBS and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100) overnight at room temperature. A Dylight-conjugated secondary antibody was used. F-actin stained with red phallodin (red) and nuclei stained with Hoechst (blue).
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ab5622 staining Thyroid Hormone Receptor beta in L6 cells (right) compared to negative control (left) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with forrmalin, permeabilized with 0.1% Triton X-100 in TBS and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100) overnight at 4°C. A Dylight-conjugated secondary antibody was used. F-actin stained with red phallodin (red) and nuclei stained with Hoechst (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (24)
ab5622 has been referenced in 24 publications.
- Ballester-Rosado CJ et al. A Role for Insulin-like Growth Factor 1 in the Generation of Epileptic Spasms in a murine model. Ann Neurol 92:45-60 (2022). PubMed: 35467038
- Kweon H et al. Excitatory neuronal CHD8 in the regulation of neocortical development and sensory-motor behaviors. Cell Rep 34:108780 (2021). PubMed: 33626347
- Chen X et al. Thyroid Hormone-Regulated Expression of Period2 Promotes Liver Urate Production. Front Cell Dev Biol 9:636802 (2021). PubMed: 33869182
- Ping S et al. Brain-derived CCR5 Contributes to Neuroprotection and Brain Repair after Experimental Stroke. Aging Dis 12:72-92 (2021). PubMed: 33532129
- Teoh J et al. Arfgef1 haploinsufficiency in mice alters neuronal endosome composition and decreases membrane surface postsynaptic GABAA receptors. Neurobiol Dis 134:104632 (2020). PubMed: 31678406