Anti-TopBP1 antibody (ab105109)
Key features and details
- Rabbit polyclonal to TopBP1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TopBP1 antibody
See all TopBP1 primary antibodies -
Description
Rabbit polyclonal to TopBP1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rabbit, Horse, Cow, Dog, Xenopus laevis, Macaque monkey, Gorilla, Chinese hamster -
Immunogen
Synthetic peptide corresponding to Human TopBP1 aa 800-900 conjugated to keyhole limpet haemocyanin.
(Peptide available asab127877) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab105109 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 171 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 171 kDa). |
Target
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Function
Required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR. -
Tissue specificity
Highly expressed in heart, brain, placenta, lung and kidney. -
Sequence similarities
Contains 8 BRCT domains. -
Post-translational
modificationsPhosphorylated on serine and threonine residues in response to X-ray irradiation.
Ubiquitinated and degraded by the proteasome. X-ray irradiation reduces ubiquitination. -
Cellular localization
Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Chromosome. Detected on unpaired autosomes in meiotic prophase cells. Detected on X and Y chromosomes during later stages of prophase. Colocalizes with ATR and H2AFX at unsynapsed chromosome cores during prophase (By similarity). Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation. Localizes to sites of DNA damage in a H2AX- independent manner. - Information by UniProt
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Database links
- Entrez Gene: 11073 Human
- Entrez Gene: 235559 Mouse
- Omim: 607760 Human
- SwissProt: Q92547 Human
- SwissProt: Q6ZQF0 Mouse
- Unigene: 593379 Human
- Unigene: 259893 Mouse
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Alternative names
- DNA topoisomerase 2 binding protein 1 antibody
- DNA topoisomerase 2-binding protein 1 antibody
- DNA topoisomerase II binding protein 1 antibody
see all
Images
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All lanes : Anti-TopBP1 antibody (ab105109) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 5 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 171 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: 120 kDa, 42 kDa, 56 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute -
All lanes : Anti-TopBP1 antibody (ab105109) at 0.5 µg/ml
All lanes : Mouse ES whole cell lysate
Lysates/proteins at 200 µg per lane.
Secondary
All lanes : HRP Goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 171 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Additional bands at: 145 kDa (possible non-specific binding), 85 kDa (possible non-specific binding)
Exposure time: 30 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab105109 has been referenced in 2 publications.
- Hirota T et al. SETDB1 Links the Meiotic DNA Damage Response to Sex Chromosome Silencing in Mice. Dev Cell 47:645-659.e6 (2018). PubMed: 30393076
- Vasileva A et al. The DNA damage checkpoint protein RAD9A is essential for male meiosis in the mouse. J Cell Sci 126:3927-38 (2013). PubMed: 23788429