Anti-Topoisomerase II alpha antibody (ab12318)
Key features and details
- Rabbit polyclonal to Topoisomerase II alpha
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-Topoisomerase II alpha antibody
See all Topoisomerase II alpha primary antibodies -
Description
Rabbit polyclonal to Topoisomerase II alpha -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide representing a portion of human DNA topoisomerase II, alpha isozyme encoded within exon 35 (LocusLink ID 7153).
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Positive control
- HeLa cells. IHC-P: FFPE human testis tissue sections.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified using the peptide immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab12318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. -
Sequence similarities
Belongs to the type II topoisomerase family. -
Post-translational
modificationsPhosphorylation has no effect on catalytic activity. -
Cellular localization
Cytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm. - Information by UniProt
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Database links
- Entrez Gene: 7153 Human
- Entrez Gene: 21973 Mouse
- Omim: 126430 Human
- SwissProt: P11388 Human
- SwissProt: Q01320 Mouse
- Unigene: 156346 Human
- Unigene: 4237 Mouse
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Alternative names
- alpha isozyme antibody
- ATP hydrolyzing DNA topoisomerase II alfa antibody
- DNA gyrase antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human laryangeal squamous cell carcinoma labelling Topoisomerase II alpha with ab12318 at 1/1000 (1µg/ml). Detection: DAB.
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Detection of Human Topo II Alpha by Western Blot and Immunoprecipitation.
Samples:
Whole cell lysate (25 mcg/lane for A; 0.5 mg for IP in B; 25 mcg for Input in B) from HeLa cells. Antibodies: Affinity purified rabbit anti-Topo II alpha antibody BL983 used at the indicated concentrations for WB (A) and IP (B). Control antibody (Ctrl. in A) and antibody for WB of immunoprecipitated Topo II alpha was a monoclonal antibody to Topo II alpha from another source. Detection: Chemiluminescence with exposure times of 5 minutes (A) or 30 seconds (B).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab12318 has been referenced in 15 publications.
- Mackay RP et al. YM155 Induces DNA Damage and Cell Death in Anaplastic Thyroid Cancer Cells by Inhibiting DNA Topoisomerase IIα at the ATP-Binding Site. Mol Cancer Ther 21:925-935 (2022). PubMed: 35405742
- Yang H et al. GINS1 promotes the proliferation and migration of glioma cells through USP15-mediated deubiquitination of TOP2A. iScience 25:104952 (2022). PubMed: 36065190
- Daiß JL et al. The human RNA polymerase I structure reveals an HMG-like docking domain specific to metazoans. Life Sci Alliance 5:N/A (2022). PubMed: 36271492
- Reale A et al. TOP2A expression predicts responsiveness to carfilzomib in myeloma and informs novel combinatorial strategies for enhanced proteasome inhibitor cell killing. Leuk Lymphoma 62:337-347 (2021). PubMed: 33131357
- Busatto FF et al. Functions of the CSB Protein at Topoisomerase 2 Inhibitors-Induced DNA Lesions. Front Cell Dev Biol 9:727836 (2021). PubMed: 34746125