Overview

  • Product name
    Anti-Treponema pallidum antibody
    See all Treponema pallidum primary antibodies
  • Description
    Rabbit polyclonal to Treponema pallidum
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Immunogen

    Full length native protein (purified) corresponding to Treponema pallidum.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    >95% pure. Sodium sulfate precipitation and DEAE chromatography.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/3000.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    Treponema pallidum is the causative agent of syphilis. It is a gram negative spirochete, a helical to sinusoidal bacterium with outer and cytoplasmic membranes, a thin peptidoglycan layer, and periplasmic flagella.
  • Alternative names
    • T. pallidum antibody

Images

  • Immunohistochemical analysis of HIV-infected syphilitic skin tissue, staining Treponema pallidum with ab20923.

    Antigen retrieval was performed before blocking with normal horse serum and 2% BSA. Samples were incubated with primary antibody overnight at 4°C. A biotinylated horse anti-rabbit IgG was used as the secondary antibody. Staining was detected using AEC.

References

This product has been referenced in:
  • Stary G  et al. Host defense mechanisms in secondary syphilitic lesions: a role for IFN-gamma-/IL-17-producing CD8+ T cells? Am J Pathol 177:2421-32 (2010). IHC-P . Read more (PubMed: 20889558) »
  • Günther U  et al. In vivo diagnosis of intestinal spirochaetosis by confocal endomicroscopy. Gut 57:1331-3 (2008). Read more (PubMed: 18719143) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for your email and sorry for the delay in getting back to you.

We have unfortunate not used the anti-Treponema pallidum antibody (ab20923) with the reagents you have described. I can say we have used the antibody in a 1/100 dilution in staining of paraffin embedded tissue sections. However, this is likely to require further optimisation when used in your particular tissues and protocol to obtain the best results.

I would just like to point out that the reagents we provide are all intended for research and development purposes only. They are not suitable for diagnostic or clinical purposes.

I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.

Read More

Answer



Unfortunately, we do not sell a positive control slide either for this antibody.

I cannot guarantee that this is a reliable source for the slides, but I found a company via the internetwhich can actually slides which are suitable for immunohistostaining and not only for Silver Staining:

http://www.newcomersupply.com/product/spirochete-treponema-hyodysenteriae


Read More

Question
Answer

Merci de nous avoir contactés.

Nous sommes désolés d'apprendre que le produit ab20095que vous avez reçu ne fonctionne pas comme attendu.

Comme convenu j'ai procédé à l'envoi d'une unité de ab20923; le numéro de commande de remplacement gratuit de ce produit est *********. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.

La publication dont je vous ai parléesur les différentes conditions de démasquage d'antigène est : Emoto et al., J Histochem Cytochem. 2005 Nov ;53 (11) : 1311-21 (Table 2).

N'hésitez pas à nous contacter lors d'une prochaine occasion.

Read More

Question
Answer

Thank you for your phone call yesterday and for your questions about ab20923. I have heard back from the laboratory that produces ab20923, and I was told that they recommend using pH 6 citrate buffer for antigen retrieval. Please let me know if you have further questions or if there is anything else that I can help you with.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up