Product nameAnti-Treponema pallidum antibody
See all Treponema pallidum primary antibodies
DescriptionRabbit polyclonal to Treponema pallidum
Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
Full length native protein (purified) corresponding to Treponema pallidum.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
PurityProtein A purified
Purification notes>95% pure. Sodium sulfate precipitation and DEAE chromatography.
Our Abpromise guarantee covers the use of ab20923 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceTreponema pallidum is the causative agent of syphilis. It is a gram negative spirochete, a helical to sinusoidal bacterium with outer and cytoplasmic membranes, a thin peptidoglycan layer, and periplasmic flagella.
- T. pallidum antibody
Immunohistochemical analysis of HIV-infected syphilitic skin tissue, staining Treponema pallidum with ab20923.
Antigen retrieval was performed before blocking with normal horse serum and 2% BSA. Samples were incubated with primary antibody overnight at 4°C. A biotinylated horse anti-rabbit IgG was used as the secondary antibody. Staining was detected using AEC.
This product has been referenced in:
- Stary G et al. Host defense mechanisms in secondary syphilitic lesions: a role for IFN-gamma-/IL-17-producing CD8+ T cells? Am J Pathol 177:2421-32 (2010). IHC-P . Read more (PubMed: 20889558) »
- Günther U et al. In vivo diagnosis of intestinal spirochaetosis by confocal endomicroscopy. Gut 57:1331-3 (2008). Read more (PubMed: 18719143) »