Anti-Trichohyalin antibody [AE15] (ab58755)
Key features and details
- Mouse monoclonal [AE15] to Trichohyalin
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Trichohyalin antibody [AE15]
See all Trichohyalin primary antibodies -
Description
Mouse monoclonal [AE15] to Trichohyalin -
Host species
Mouse -
Specificity
This antibody recognises a 220K trichohyalin granular protein, a marker for hair follicular differentiation. -
Tested applications
Suitable for: WB, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Tissue, cells or virus corresponding to Human Trichohyalin.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
AE15 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab58755 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 220 kDa.
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IHC-P | (1) |
Use at an assay dependent concentration. PubMed: 19038788
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IHC-Fr | (1) |
Use at an assay dependent concentration. PubMed: 19786578
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Notes |
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WB
1/1000. Predicted molecular weight: 220 kDa. |
IHC-P
Use at an assay dependent concentration. PubMed: 19038788 |
IHC-Fr
Use at an assay dependent concentration. PubMed: 19786578 |
Target
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Function
Intermediate filament-associated protein that associates in regular arrays with keratin intermediate filaments (KIF) of the inner root sheath cells of the hair follicle and the granular layer of the epidermis. It later becomes cross-linked to KIF by isodipeptide bonds. It may serve as scaffold protein, together with involucrin, in the organization of the cell envelope or even anchor the cell envelope to the KIF network. It may be involved in its own calcium-dependent postsynthetic processing during terminal differentiation. -
Tissue specificity
Found in the hard keratinizing tissues such as the inner root sheath (IRS) of hair follicles and medulla, and in the filiform papillae of dorsal tongue epithelium. -
Sequence similarities
Belongs to the S100-fused protein family.
Contains 2 EF-hand domains. -
Developmental stage
Expressed during late differentiation of the epidermis. -
Domain
Consists of nine domains. Domain 1 contains two EF-hand calcium-binding domains. Domains 2-4, 6, and 8 are almost entirely alpha-helical, configured as a series of peptide repeats of varying regularity, and are thought to form a single-stranded alpha-helical rod stabilized by ionic interactions. Domain 6 is the most regular and may bind KIF directly by ionic interactions. Domains 5 and 7 are less well organized and may induce folds in the molecule. Domain 9 contains the C-terminus, conserved among different species. -
Post-translational
modificationsSubstrate of transglutaminase. Some 200 arginines are probably converted to citrullines by peptidylarginine deimidase. - Information by UniProt
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Database links
- Entrez Gene: 7062 Human
- Entrez Gene: 99681 Mouse
- SwissProt: Q07283 Human
- Unigene: 432416 Human
- Unigene: 553300 Human
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Alternative names
- 220K trichohyalin granular protein antibody
- Hair follicle protein AHF antibody
- MGC157889 antibody
see all
Images
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Anti-Trichohyalin antibody [AE15] (ab58755) at 1 µg/ml + Lung (Human) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 220 kDa
Observed band size: 220 kDa
Additional bands at: 165 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab58755 has been referenced in 15 publications.
- Tripurani SK et al. Suppression of Wnt/ß-catenin signaling by EGF receptor is required for hair follicle development. Mol Biol Cell 29:2784-2799 (2018). PubMed: 30188763
- Sardella C et al. Delayed Hair Follicle Morphogenesis and Hair Follicle Dystrophy in a Lipoatrophy Mouse Model of Pparg Total Deletion. J Invest Dermatol 138:500-510 (2018). PubMed: 28964716
- Leng X et al. Dissociated skin cells regenerate hair follicles in a microwound, "The Punch Assay". Exp Dermatol N/A:N/A (2018). PubMed: 30033549
- Furukawa S et al. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol 30:79-107 (2017). IHC ; Rabbit . PubMed: 28190929
- Imsland F et al. Regulatory mutations in TBX3 disrupt asymmetric hair pigmentation that underlies Dun camouflage color in horses. Nat Genet 48:152-8 (2016). PubMed: 26691985