Overview

  • Product name
    Anti-Troponin I fast skeletal muscle antibody [2F12G2]
    See all Troponin I fast skeletal muscle primary antibodies
  • Description
    Mouse monoclonal [2F12G2] to Troponin I fast skeletal muscle
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ELISA, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified full-length recombinant Human Troponin I fast skeletal muscle expressed in E. Coli.

  • Positive control
    • Recombinant Human TNNI2 protein (AA: 1-182), HEK293 and TNNI2 (AA: 1-182)-hIgGFc transfected HEK293 cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified from tissue culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    2F12G2
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
ELISA 1/10000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/200 - 1/1000.

Target

  • Function
    Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Involvement in disease
    Defects in TNNI2 are a cause of distal arthrogryposis type 2B (DA2B) [MIM:601680]; also known as arthrogryposis multiplex congenita, distal, type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin.
  • Sequence similarities
    Belongs to the troponin I family.
  • Information by UniProt
  • Database links
  • Alternative names
    • AMCD 2B antibody
    • AMCD2B antibody
    • DA 2B antibody
    • DA2B antibody
    • fast skeletal muscle antibody
    • Fast twitch skeletal muscle troponin I antibody
    • fast-twitch isoform antibody
    • FSSV antibody
    • fsTnI antibody
    • TNNI 2 antibody
    • Tnni2 antibody
    • TNNI2_HUMAN antibody
    • tro antibody
    • Troponin I antibody
    • Troponin I fast skeletal muscle antibody
    • Troponin I fast twitch 2 antibody
    • Troponin I fast twitch isoform antibody
    • Troponin I fast twitch skeletal muscle isoform antibody
    • Troponin I skeletal fast antibody
    • Troponin I type 2 (skeletal fast) antibody
    • Troponin I type 2 antibody
    see all

Images

  • Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824) at 1/500 dilution + Recombinant Human TNNI2 protein (AA: 1-182)

    Predicted band size: 21 kDa

  • All lanes : Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : TNNI2 (AA: 1-182)-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 21 kDa

  • Example of standard curves obtained using ab126824. Control antigen 100ng (black), antigen 10ng (purple), antigen 50ng (blue), antigen 100ng (red)
  • Immunofluorescence analysis of Hela cells using ab126824 at 1/200 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Flow cytometric analysis of NIH/3T3 cells using ab126824 AT 1/200 (green) and negative control (red).

References

ab126824 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.

I have confirmed that the immunogen for ab126824 is full-length recombinant protein, not a fragment. The epitope has not been mapped. The trouble you are having with the sandwich ELISA may be a consequence of the two antibodies recognizing overlapping epitopes but it is more likely that one of the antibodies is failing. If you can, try checking for this with an indirect ELISA against antigen coated directly to the plate, followed by blocking with BSA, the antibody, and a secondary conjugated antibody.

For the sandwich, I recommend capturing with the monoclonal and detecting with the polyclonal ab123876, assuming you have an anti-rabbit secondary. Ideally, it should be absorbed to remove reactivity with mouse IgG1, the isotype of ab126824.

I hope this is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for your inquiry.


The antibody’s concentration is 1 mg/ml.


I hope this information helps. Please contact us with any other questions.

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