Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824)
Key features and details
- Mouse monoclonal [2F12G2] to Troponin I fast skeletal muscle
- Suitable for: WB, ELISA, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
-
Product name
Anti-Troponin I fast skeletal muscle antibody [2F12G2]
See all Troponin I fast skeletal muscle primary antibodies -
Description
Mouse monoclonal [2F12G2] to Troponin I fast skeletal muscle -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISA, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Purified full-length recombinant Human Troponin I fast skeletal muscle expressed in E. Coli.
-
Positive control
- Recombinant Human TNNI2 protein (AA: 1-182), HEK293 and TNNI2 (AA: 1-182)-hIgGFc transfected HEK293 cell lysates.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2F12G2 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
|
|
ELISA |
1/10000.
|
|
Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
ICC/IF |
1/200 - 1/1000.
|
Notes |
---|
WB
1/500 - 1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
ELISA
1/10000. |
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/200 - 1/1000. |
Target
-
Function
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. -
Involvement in disease
Defects in TNNI2 are a cause of distal arthrogryposis type 2B (DA2B) [MIM:601680]; also known as arthrogryposis multiplex congenita, distal, type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin. -
Sequence similarities
Belongs to the troponin I family. - Information by UniProt
-
Database links
- Entrez Gene: 7136 Human
- Entrez Gene: 21953 Mouse
- Omim: 191043 Human
- SwissProt: P48788 Human
- SwissProt: P13412 Mouse
- Unigene: 523403 Human
- Unigene: 39469 Mouse
-
Alternative names
- AMCD 2B antibody
- AMCD2B antibody
- DA 2B antibody
see all
Images
-
Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824) at 1/500 dilution + Recombinant Human TNNI2 protein (AA: 1-182)
Predicted band size: 21 kDa -
All lanes : Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : TNNI2 (AA: 1-182)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 21 kDa -
Example of standard curves obtained using ab126824. Control antigen 100ng (black), antigen 10ng (purple), antigen 50ng (blue), antigen 100ng (red)
-
Immunofluorescence analysis of Hela cells using ab126824 at 1/200 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
-
Flow cytometric analysis of NIH/3T3 cells using ab126824 AT 1/200 (green) and negative control (red).
Protocols
Datasheets and documents
-
Datasheet download
References (1)
ab126824 has been referenced in 1 publication.
- Boularaoui SM et al. Efficient transdifferentiation of human dermal fibroblasts into skeletal muscle. J Tissue Eng Regen Med 12:e918-e936 (2018). PubMed: 28101909