Recombinant Anti-UBLCP1 antibody [EPR11923] (ab176340)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11923] to UBLCP1
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-UBLCP1 antibody [EPR11923]
See all UBLCP1 primary antibodies -
Description
Rabbit monoclonal [EPR11923] to UBLCP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293T, HeLa, and K562 cell lysates. HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11923 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab176340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
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ICC/IF |
1/50 - 1/100.
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IP |
1/10 - 1/100.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa. |
ICC/IF
1/50 - 1/100. |
IP
1/10 - 1/100. |
Target
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Function
Dephosphorylates 26S nuclear proteasomes, thereby decreasing their proteolytic activity. The dephosphorylation may prevent assembly of the core and regulatory particles (CP and RP) into mature 26S proteasome. -
Tissue specificity
Broadly expressed, with highest levels in placenta, lung, testis and ovary. Up-regulated in tumor tissues. -
Sequence similarities
Contains 1 FCP1 homology domain.
Contains 1 ubiquitin-like domain. -
Domain
The Ubiquitin-like domain mediates interaction with proteasomes. -
Cellular localization
Nucleus. Colocalizes with nuclear proteasomes. - Information by UniProt
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Database links
- Entrez Gene: 134510 Human
- Omim: 609867 Human
- SwissProt: Q8WVY7 Human
- Unigene: 591733 Human
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Alternative names
- CPUB1 antibody
- CTD like phosphatase domain containing protein antibody
- CTD phosphatase like with ubiquitin domain 1 antibody
see all
Images
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Immunofluorescence analysis of HeLa cells labeling UBLCP1 with ab176340 at 1/50 dilution.
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All lanes : Anti-UBLCP1 antibody [EPR11923] (ab176340) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 37 kDa -
Western blot analysis on immunoprecipitation pellet from K562 cell lysate using ab176340 at 1/10 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab176340 has been referenced in 1 publication.
- Sun S et al. Phosphatase UBLCP1 controls proteasome assembly. Open Biol 7:N/A (2017). PubMed: 28539385